Li Yuan-Yuan, Xu Ting-Ting, Ai Zhe, Wei Lu-Lu, Ma Fei
School of Ecology and Environment, Ningxia University, Yinchuan 750021, China.
Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China, Ningxia University, Yinchuan 750021, China.
Huan Jing Ke Xue. 2023 Apr 8;44(4):2304-2314. doi: 10.13227/j.hjkx.202204165.
, as the main dominant plant in the alpine and subalpine regions of northern China, is an important component of the local ecosystem. However, few studies have paid attention to its impact on the soil ecosystem and its response to environmental changes. Thus, in this study, we used high-throughput sequencing technology to investigate diversity and predictive function of rhizosphere and bulk soil bacteria communities of from different altitudes. The results indicated that 43 phyla, 112 classes, 251 orders, 324 families, and 542 genera were obtained from the soil. The dominant phyla in all sample sites were Proteobacteria, Acidobacteria, and Actinobacteria. There were significant differences in bacterial diversity index and community structure between the rhizosphere and bulk soil at the same altitude, whereas the differences across altitudes were insignificant. PICRUSt analysis showed that the functional gene families were mainly related to 29 sub-functions, including amino acid metabolism, carbohydrate metabolism, and metabolism of cofactors and vitamins, and the abundance of metabolism was highest. There were significant correlations between the relative abundances of genes involved in the level Ⅱ metabolic pathway of bacteria and phylum-level taxa, such as Proteobacteria, Acidobacteria, and Chloroflexi. The predicted functional compositions of soil bacteria also showed a significantly positive correlation with the dissimilarity in bacterial community structure, indicating that there was a strong relationship between bacterial community structure and functional genes. This study preliminarily discussed the characteristics and functional prediction analysis of bacterial communities in the rhizosphere and bulk soil of at different altitudinal gradients, which provided data support for the ecological effects of constructive plants and their responses to environmental changes in high altitude areas.
作为中国北方高寒和亚高寒地区的主要优势植物，是当地生态系统的重要组成部分。然而，很少有研究关注其对土壤生态系统的影响及其对环境变化的响应。因此，在本研究中，我们使用高通量测序技术研究了不同海拔高度的[植物名称]根际和非根际土壤细菌群落的多样性和预测功能。结果表明，从土壤中获得了43个门、112个纲、251个目、324个科和542个属。所有采样点的优势门为变形菌门、酸杆菌门和放线菌门。同一海拔高度的根际和非根际土壤细菌多样性指数和群落结构存在显著差异，而不同海拔高度之间的差异不显著。PICRUSt分析表明，功能基因家族主要与29个亚功能相关，包括氨基酸代谢、碳水化合物代谢以及辅因子和维生素代谢，其中代谢功能的丰度最高。细菌Ⅱ级代谢途径中涉及的基因相对丰度与变形菌门、酸杆菌门和绿弯菌门等门水平的分类群之间存在显著相关性。土壤细菌的预测功能组成也与细菌群落结构的差异呈显著正相关，表明细菌群落结构与功能基因之间存在很强的关系。本研究初步探讨了不同海拔梯度下[植物名称]根际和非根际土壤细菌群落的特征及功能预测分析，为高海拔地区建群植物的生态效应及其对环境变化的响应提供了数据支持。
