VitaVitro Biotech Co., Ltd., Shenzhen, Guangdong, China.
RVT Australia, Cairns, QLD, Australia.
Methods Mol Biol. 2023;2647:183-195. doi: 10.1007/978-1-0716-3064-8_9.
Somatic cell nuclear transfer (SCNT) in pigs is a promising technology in biomedical research by association with transgenesis for xenotransplantation and disease modeling technologies. Handmade cloning (HMC) is a simplified SCNT method that does not require micromanipulators and facilitates the generation of cloned embryos in large quantities. As a result of HMC fine-tuning for porcine-specific requirements of both oocytes and embryos, HMC has become uniquely efficient (>40% blastocyst rate, 80-90% pregnancy rates, 6-7 healthy offspring per farrowing, and with negligible losses and malformations). Therefore, this chapter describes our HMC protocol to obtain cloned pigs.
体细胞核移植（SCNT）在猪中是一种有前途的生物技术，可与转基因、异种移植和疾病建模技术相关联。手工克隆（HMC）是一种简化的 SCNT 方法，不需要微操作器，并且有利于大量生成克隆胚胎。由于 HMC 针对卵母细胞和胚胎的猪特异性要求进行了精细调整，因此 HMC 变得非常高效（>40%的囊胚率、80-90%的妊娠率、每窝 6-7 头健康后代，几乎没有损失和畸形）。因此，本章描述了我们获得克隆猪的 HMC 方案。
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