Institut für Fortpflanzung landwirtschaftlicher Nutztiere Schönow e.V, Bernau bei Berlin, Germany.
Reproductive Sciences Unit, Toronto Zoo, Toronto, ON, Canada.
Methods Mol Biol. 2023;2647:259-268. doi: 10.1007/978-1-0716-3064-8_14.
Interspecies somatic cell nuclear transfer (iSCNT) contributes to the preservation of endangered species, albeit nuclear-mitochondrial incompatibilities constrain its application. iSCNT, coupled with ooplasm transfer (iSCNT-OT), has the potential to overcome the challenges associated with species- and genus-specific differences in nuclear-mitochondrial communication. Our iSCNT-OT protocol combines the transfer of both bison (Bison bison bison) somatic cell and oocyte ooplasm by a two-step electrofusion into bovine (Bos taurus) enucleated oocytes. The procedures described herein could be used in further studies to determine the effects of crosstalk between nuclear and ooplasmic components in embryos carrying genomes from different species.
种间体细胞核移植（iSCNT）有助于保护濒危物种，尽管核-线粒体不相容性限制了其应用。iSCNT 与卵质转移（iSCNT-OT）相结合，有可能克服与核-线粒体通讯的物种和属特异性差异相关的挑战。我们的 iSCNT-OT 方案通过两步电融合将野牛（Bison bison bison）体细胞和卵母细胞卵质一起转移到牛（Bos taurus）去核卵母细胞中。本文所述的程序可用于进一步研究，以确定携带不同物种基因组的胚胎中核和卵质成分之间串扰的影响。
