使用包被透明带和去透明带卵母细胞的马体细胞核移植。

Horse Somatic Cell Nuclear Transfer Using Zona Pellucida-Enclosed and Zona-Free Oocytes.

机构信息

Universidad de Buenos Aires, Facultad de Agronomía, Departamento de Producción Animal, Buenos Aires, Laboratorio Biotecnología Animal (LabBA), Buenos Aires, Argentina.

Instituto de Investigaciones en Producción Animal (INPA), CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina.

出版信息

Methods Mol Biol. 2023;2647:269-281. doi: 10.1007/978-1-0716-3064-8_15.

DOI:10.1007/978-1-0716-3064-8_15

PMID:37041341

Abstract

Horse cloning by somatic cell nuclear transfer (SCNT) is an attractive scientific and commercial endeavor. Moreover, SCNT allows generating genetically identical animals from elite, aged, castrated, or deceased equine donors. Several variations in the horse SCNT method have been described, which may be useful for specific applications. This chapter describes a detailed protocol for horse cloning, thus including SCNT protocols using zona pellucida (ZP)-enclosed or ZP-free oocytes for enucleation. These SCNT protocols are under routine use for commercial equine cloning.

摘要

体细胞核移植（SCNT）克隆马是一项具有吸引力的科学和商业活动。此外，SCNT 可使来自优秀、高龄、去势或死亡的马供体产生遗传上相同的动物。马 SCNT 方法有几种变化，这些变化可能对特定应用有用。本章描述了马克隆的详细方案，包括使用有或无透明带的卵母细胞进行去核的 SCNT 方案。这些 SCNT 方案在商业马克隆中常规使用。