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蛋氨酸脑啡肽上调巨噬细胞抗甲型流感病毒感染的蛋白质组学分析。

Proteomics analysis of methionine enkephalin upregulated macrophages against infection by the influenza-A virus.

作者信息

Fu Wenrui, Xie Zifeng, Bai Mei, Zhang Zhen, Zhao Yuanlong, Tian Jing

机构信息

Graduate College, Jinzhou Medical University, Jinzhou, 121000, China.

First Clinical Medical College, Jinzhou Medical University, Jinzhou, 121000, China.

出版信息

Proteome Sci. 2023 Apr 11;21(1):4. doi: 10.1186/s12953-023-00205-w.

DOI:10.1186/s12953-023-00205-w
PMID:37041527
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10088144/
Abstract

Macrophages have a vital role in phagocytosis and antiviral effect against invading influenza viruses. Previously, we found that methionine enkephalin (MENK) inhibited influenza virus infection by upregulating the "antiviral state" of macrophages. To investigate the immunoregulatory mechanism of action of MENK on macrophages, we employed proteomic analysis to identify differentially expressed proteins (DEPs) between macrophages infected with the influenza-A virus and cells infected with the influenza-A virus after pretreatment with MENK. A total of 215 DEPs were identified: 164 proteins had upregulated expression and 51 proteins had downregulated expression. Proteomics analysis showed that DEPs were highly enriched in "cytokine-cytokine receptor interaction", "phagosome", and "complement and coagulation cascades pathway". Proteomics analysis revealed that MENK could be an immune modulator or prophylactic for the prevention and treatment of influenza. MENK promoted the polarization of M1 macrophages, activated inflammatory responses, and enhanced phagocytosis and killing function by upregulating opsonizing receptors.

摘要

巨噬细胞在吞噬作用以及针对入侵流感病毒的抗病毒效应中发挥着至关重要的作用。此前，我们发现甲硫氨酸脑啡肽（MENK）通过上调巨噬细胞的“抗病毒状态”来抑制流感病毒感染。为了探究MENK对巨噬细胞的免疫调节作用机制，我们采用蛋白质组学分析来鉴定甲型流感病毒感染的巨噬细胞与经MENK预处理后感染甲型流感病毒的细胞之间的差异表达蛋白（DEP）。共鉴定出215种DEP：164种蛋白表达上调，51种蛋白表达下调。蛋白质组学分析表明，DEP在“细胞因子-细胞因子受体相互作用”、“吞噬体”和“补体与凝血级联途径”中高度富集。蛋白质组学分析显示，MENK可能是预防和治疗流感的免疫调节剂或预防剂。MENK通过上调调理素受体促进M1巨噬细胞极化，激活炎症反应，并增强吞噬和杀伤功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9e9/10088144/25a459d0bd4d/12953_2023_205_Fig10_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9e9/10088144/646932cb0b79/12953_2023_205_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9e9/10088144/25a459d0bd4d/12953_2023_205_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9e9/10088144/62c5cbb390d5/12953_2023_205_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9e9/10088144/d61651591bdf/12953_2023_205_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9e9/10088144/12877607d295/12953_2023_205_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9e9/10088144/c6389511c66d/12953_2023_205_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9e9/10088144/3fa8b22c59d6/12953_2023_205_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9e9/10088144/58e296e2894d/12953_2023_205_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9e9/10088144/f7c825d83919/12953_2023_205_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9e9/10088144/7f1ab4cad204/12953_2023_205_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9e9/10088144/646932cb0b79/12953_2023_205_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9e9/10088144/25a459d0bd4d/12953_2023_205_Fig10_HTML.jpg

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Am J Respir Cell Mol Biol. 2023 Feb;68(2):213-227. doi: 10.1165/rcmb.2022-0056OC.
3
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J Cell Biol. 2022 Dec 5;221(12). doi: 10.1083/jcb.202201114. Epub 2022 Oct 5.
4
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Front Microbiol. 2022 Aug 8;13:937192. doi: 10.3389/fmicb.2022.937192. eCollection 2022.
5
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6
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7
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Front Immunol. 2022 Jan 20;12:826621. doi: 10.3389/fimmu.2021.826621. eCollection 2021.
8
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Front Immunol. 2022 Jan 18;12:798719. doi: 10.3389/fimmu.2021.798719. eCollection 2021.
9
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Front Immunol. 2021 Jul 13;12:711997. doi: 10.3389/fimmu.2021.711997. eCollection 2021.
10
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