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高通量测序法对自然发酵酸菜微生物群的表征

Characterization of microbiota of naturally fermented sauerkraut by high-throughput sequencing.

作者信息

Zhang Shuang, Zhang Yichen, Wu Lihong, Zhang Lili, Wang Song

机构信息

Food Science College, Northeast Agricultural University, Harbin, 150030 People's Republic of China.

Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, 150030 People's Republic of China.

出版信息

Food Sci Biotechnol. 2022 Dec 23;32(6):855-862. doi: 10.1007/s10068-022-01221-w. eCollection 2023 May.

Abstract

is a traditionally fermented cabbage, with a unique taste and beneficial properties, in northeast China. The taste and flavor of vary from region to region, owing to the differences in microorganisms. Illumina MiSeq sequencing was used to identify and quantify the microbial community composition of the broth and leaves of the naturally fermented collected from northeast China. The alpha and beta diversity of the samples from three areas in Heilongjiang province shown that the complexity of bacterial diversity of the three samples was C, A and B in turn. The widely existed in fermented , of these, , subsp. , subsp. , and were more abundant in the leaves than in fermentation broth. Other genera of lactic acid bacteria and , which have potential probiotic properties, were also present. However, some harmful bacteria such as and were also detected.

摘要

是中国东北地区一种传统发酵的卷心菜,具有独特的味道和有益特性。由于微生物的差异,其味道和风味因地区而异。使用Illumina MiSeq测序来鉴定和量化从中国东北地区采集的自然发酵的肉汤和叶子中的微生物群落组成。黑龙江省三个地区样本的α和β多样性表明,三个样本的细菌多样性复杂性依次为C、A和B。在发酵的中广泛存在,其中,、亚种、亚种和在叶子中比在发酵液中更丰富。还存在其他具有潜在益生菌特性的乳酸菌属和。然而,也检测到了一些有害细菌,如和。

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