Smith-Buijs C M, Borgsteede F H
Res Vet Sci. 1986 Jan;40(1):4-7.
An in vitro egg development assay was used to test the resistance of eggs from a benzimidazole susceptible and a resistant strain of Haemonchus contortus to four benzimidazoles. Storage of eggs contained in faecal samples at 4 degrees C for four, 24 or 72 hours did not appear to impair the results from the in vitro egg development assay or alter the LD50. Storage for one week, however, proved to be lethal for the eggs. The average LD50 values for thiabendazole, albendazole, mebendazole and cambendazole were 0.06, 0.07, 0.96 and 2.47 ppm for the susceptible strain. For the resistant strain the values averaged 0.26, 0.24, 1.07 and 5.11 ppm.
采用体外虫卵发育试验，检测了来自苯并咪唑敏感和抗性捻转血矛线虫株的虫卵对四种苯并咪唑的抗性。粪便样本中的虫卵在4℃下储存4、24或72小时，似乎并未影响体外虫卵发育试验的结果，也未改变半数致死剂量（LD50）。然而，储存一周对虫卵来说是致命的。对于敏感株，噻苯达唑、阿苯达唑、甲苯达唑和坎苯达唑的平均LD50值分别为0.06、0.07、0.96和2.47 ppm。对于抗性株，这些值平均为0.26、0.24、1.07和5.11 ppm。
