Li Jing-Yang, Godeiro Nerivania Nunes, Gao Yan, Bu Yun, Li Kai
School of Life Sciences, East China Normal University, Shanghai 200241, China.
atural History Research Center, Shanghai Natural History Museum, Shanghai Science & Technology Museum, Shanghai, 200041, China.
Zootaxa. 2022 Dec 22;5222(5):467-477. doi: 10.11646/zootaxa.5222.5.5.
Members of the order Poduromorpha are mostly small and delicate, many of them are notably smaller than 1 mm. For this reason, studies at the molecular level are still scarce, but modern sequencing technologies are making it possible and the Collembola genetic database have increased significantly in the last ten years. In order to contribute to future studies at the molecular level of the group, we sequenced and annotated three mitogenomes of Tullbergiidae species from China, two of them are newly determined. The mitogenomes of Paratullbergia changfengensis, Metaphorura motuoensis and Mesaphorura yosii have 15,210 bp, 14,873 bp and 14,831 bp in length, respectively, containing 13 protein-coding genes (PCGs), 22 transfer-RNA-coding genes (tRNA), and 2 ribosomal-RNA-coding genes (rRNA) with the ancestral Pancrustacean gene order, typical for most mitogenomes of Collembola. We performed phylogenetic analyses based on 13 PCGs with protein sequences using Bayesian inference and maximum likelihood methods. Our phylogenetic analyses including all available mitogenomes of Poduromorpha and the new mitogenomes consistently confirmed the taxonomical placement of the three Chinese species within Tullbergiidae with high support. The monophyly of the family was confirmed with Onychiuridae as its sister group.
跳虫目成员大多体型微小且脆弱，其中许多明显小于1毫米。因此，分子水平的研究仍然很少，但现代测序技术使这类研究成为可能，并且在过去十年中，弹尾虫遗传数据库有了显著增加。为了推动该类群在分子水平上的未来研究，我们对来自中国的三种圆跳虫科物种的线粒体基因组进行了测序和注释，其中两种是新测定的。长丰副圆跳虫、墨脱原圆跳虫和吉氏中圆跳虫的线粒体基因组长度分别为15,210 bp、14,873 bp和14,831 bp，包含13个蛋白质编码基因（PCGs）、22个转运RNA编码基因（tRNA）和2个核糖体RNA编码基因（rRNA），具有典型的甲壳动物祖先基因排列顺序，这在大多数弹尾虫线粒体基因组中很常见。我们使用贝叶斯推断和最大似然法，基于13个PCGs的蛋白质序列进行了系统发育分析。我们的系统发育分析包括所有可用的跳虫目线粒体基因组和新的线粒体基因组，一致高度支持地确认了这三种中国物种在圆跳虫科内的分类地位。该科的单系性得到确认，其姐妹群为长角跳虫科。
