Ahmadian Mina, Maleki Kambakhsh Sara, Einollahi Nahid, Babazadeh Saber, Tofangchiha Maryam, D'Amato Giuseppe, Patini Romeo
School of Dentistry, Qazvin University of Medical Sciences, Qazvin 34199-15315, Iran.
Dental Caries Prevention Research Center, Qazvin University of Medical Sciences, Qazvin 34199-15315, Iran.
Diagnostics (Basel). 2023 Apr 3;13(7):1335. doi: 10.3390/diagnostics13071335.
This study aimed to assess the qualitative changes in the saliva during the process of primary teeth eruption. This cross-sectional study was conducted on 147 children from 2 to 48 months, of which 49 were in group A (no erupted primary teeth), 53 were in group B (at least one active erupting primary tooth), and 45 were in group C (eruption of all 20 primary teeth was completed). Salivary proteins were evaluated by sodium dodecyl sulfate electrophoresis with polyacrylamide gel, while the concentrations of salivary sodium, potassium, chloride, and calcium ions were evaluated by ion selective electrodes. The data were analyzed using ANOVA and Bonferroni tests (alpha = 0.05). The concentration of proteins with molecular weights of 20-30 KDa was significantly higher in group A, and it gradually decreased with age. The concentration of proteins with molecular weights of 50-60 KDa in group B was significantly lower than those of groups A and C. The calcium ion concentration in group A was significantly higher than that of the other groups. The concentration of potassium ions was minimal in group C. The proteins and electrolyte profiles of the subjects' saliva changed in the process of primary tooth eruption. The highest concentrations of proteins such as statherin, histatin, P-B peptide, and cystatin and the lowest concentrations of proteins such as amylase were present in group B.
本研究旨在评估乳牙萌出过程中唾液的质量变化。这项横断面研究对147名年龄在2至48个月的儿童进行，其中49名在A组（乳牙未萌出），53名在B组（至少有一颗乳牙正在萌出），45名在C组（20颗乳牙全部萌出完成）。唾液蛋白通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳进行评估，而唾液钠、钾、氯和钙离子的浓度通过离子选择性电极进行评估。数据采用方差分析和邦费罗尼检验进行分析（α = 0.05）。A组中分子量为20 - 30 kDa的蛋白质浓度显著更高，且随年龄逐渐降低。B组中分子量为50 - 60 kDa的蛋白质浓度显著低于A组和C组。A组中的钙离子浓度显著高于其他组。C组中的钾离子浓度最低。在乳牙萌出过程中，受试者唾液的蛋白质和电解质谱发生了变化。B组中存在如富组蛋白、组蛋白、P - B肽和胱抑素等蛋白质的最高浓度，以及如淀粉酶等蛋白质的最低浓度。
