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miR-125b-5p、miR-155-3p 和 miR-214-5p 及其在口腔鳞状细胞癌中的靶基因。

miR-125b-5p, miR-155-3p, and miR-214-5p and Target Gene in Oral Squamous Cell Carcinoma.

机构信息

Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 19 Jordana St., 41-808 Zabrze, Poland.

Silesia LabMed Research and Implementation Center, Medical University of Silesia in Katowice, 19 Jordana St., 41-808 Zabrze, Poland.

出版信息

Int J Mol Sci. 2023 Mar 28;24(7):6320. doi: 10.3390/ijms24076320.

DOI:10.3390/ijms24076320
PMID:37047293
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10094498/
Abstract

It is known that E2F2 (E2F transcription factor 2) plays an important role as controller in the cell cycle. This study aimed to analyse the expression of the gene and E2F2 protein and demonstrate target microRNAs (miRNAs) candidates (miR-125b-5p, miR-155-3p, and miR-214-5p) in oral squamous cell carcinoma tumour and margin samples. The study group consisted 50 patients. The gene and miRNAs expression levels were assessed by qPCR, while the E2F2 protein was assessed by ELISA. When analysing the effect of miRNAs expression on gene expression and E2F2 protein level, we observed no statistically significant correlations. miR-125b-5p was downregulated, while miR-155-3p, and miR-214-5p were upregulated in tumour samples compared to margin. We observed a difference between the miR-125b-5p expression level in smokers and non-smokers in margin samples. Furthermore, HPV-positive individuals had a significantly higher miR-125b-5p and miR-214-5p expression level compared to HPV-negative patients in tumour samples. The study result showed that the gene is not the target for analysed miRNAs in OSCC. Moreover, miR-155-3p and miR-125b-5p could play roles in the pathogenesis of OSCC. A differential expression of the analysed miRNAs was observed in response to tobacco smoke and HPV status.

摘要

已知 E2F2（E2F 转录因子 2）在细胞周期中作为控制器发挥重要作用。本研究旨在分析基因和 E2F2 蛋白的表达，并在口腔鳞状细胞癌肿瘤和边缘样本中证明候选靶 microRNAs（miRNAs）（miR-125b-5p、miR-155-3p 和 miR-214-5p）。研究组由 50 名患者组成。通过 qPCR 评估基因和 miRNAs 表达水平，通过 ELISA 评估 E2F2 蛋白。当分析 miRNA 表达对基因表达和 E2F2 蛋白水平的影响时，我们没有观察到统计学上显著的相关性。与边缘相比，肿瘤样本中 miR-125b-5p 下调，而 miR-155-3p 和 miR-214-5p 上调。我们观察到边缘样本中吸烟者和非吸烟者 miR-125b-5p 表达水平的差异。此外，与 HPV 阴性患者相比，肿瘤样本中 HPV 阳性个体的 miR-125b-5p 和 miR-214-5p 表达水平显著更高。研究结果表明，基因不是 OSCC 中分析的 miRNAs 的靶标。此外，miR-155-3p 和 miR-125b-5p 可能在 OSCC 的发病机制中发挥作用。分析的 miRNAs 的表达水平因烟草烟雾和 HPV 状态而异。

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2
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Biomedicines. 2022 Nov 23;10(12):3011. doi: 10.3390/biomedicines10123011.
3
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Future Oncol. 2022 Sep;18(29):3311-3322. doi: 10.2217/fon-2022-0448. Epub 2022 Sep 1.
4
Potential Role of miR-196a and miR-196b as Prognostic Biomarkers of Survival in Head and Neck Squamous Cell Carcinoma: A Systematic Review, Meta-Analysis and Trial Sequential Analysis.miR-196a和miR-196b作为头颈部鳞状细胞癌生存预后生物标志物的潜在作用：一项系统评价、荟萃分析和试验序贯分析
Life (Basel). 2022 Aug 19;12(8):1269. doi: 10.3390/life12081269.
5
Gene network analysis of the transcriptome impact of methylated microRNAs on oral squamous cell carcinoma.甲基化 microRNAs 对口腔鳞状细胞癌转录组影响的基因网络分析。
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6
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