Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
The Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang 212018, China.
Int J Mol Sci. 2023 Apr 3;24(7):6670. doi: 10.3390/ijms24076670.
() is a member of the gene group involved in cell fate decisions, cell proliferation and organ identity. Its function has been extensively researched in but little is known about it in Lepidoptera. To uncover the function of in the development of lepidopterans, we constructed the overexpression (Ubx) strain based on the Nistari strain of . The Ubx strain showed a small body size, transparent intersegmental membrane and abnormal posterior silk gland (PSG). In the current study, we focused on the effect of overexpression on the posterior silk gland. As the major protein product of PSG, the mRNA expression of () and () was upregulated three times in Ubx, but the protein expression of Fib-H and Fib-L was not significantly different. We speculated that the overexpression of downregulated the expression of and further caused abnormal synthesis of the spliceosome and ribosome. Abnormalities of the spliceosome and ribosome affected the synthesis of protein in the PSG and changed its morphology.
() 是参与细胞命运决定、细胞增殖和器官身份的基因家族的一员。其功能在 中已得到广泛研究，但在鳞翅目昆虫中知之甚少。为了揭示 在鳞翅目昆虫发育中的功能，我们基于 Nistari 品系的 构建了 过表达（Ubx）株系。Ubx 株系表现出体型小、节间膜透明和后丝腺（PSG）异常。在本研究中，我们专注于 过表达对后丝腺的影响。作为 PSG 的主要蛋白产物，Ubx 中 （）和 （）的 mRNA 表达上调了三倍，但 Fib-H 和 Fib-L 的蛋白表达没有显著差异。我们推测 过表达下调了 的表达，进一步导致剪接体和核糖体的异常合成。剪接体和核糖体的异常影响了 PSG 中蛋白质的合成，并改变了其形态。
