Sakaguchi Kenichiro, Kawano Kohei, Otani Yuki, Yanagawa Yojiro, Katagiri Seiji, Telfer Evelyn E
Institute of Cell Biology, School of Biological Sciences, College of Science and Engineering, University of Edinburgh, The Hugh Robson Building, 15 George Square, Edinburgh EH8 9XD, UK.
Laboratory of Theriogenology, Department of Clinical Sciences, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-ku, Sapporo 060-0818, Japan.
Animals (Basel). 2023 Mar 23;13(7):1141. doi: 10.3390/ani13071141.
The amino acid metabolism of bovine follicles during in vitro growth (IVG) was evaluated to identify potential indicators of health during culture. The bovine ovarian cortex was sliced, prepared as strips, and cultured for 6 days. Tissue samples were examined histologically before and after 6 days of culture, and the degree of follicle activation was classified as either high or low based on the number of growing secondary follicles present (high: 711; low: 01). In a separate experiment, secondary follicles (diameter range: 100200 μm) were manually isolated and cultured, and their growth was monitored for 6 days. Cultured follicles were classified as growth or degenerate based on diameter change during culture (growth: +60.574.1 μm; degenerate: -28~15.2 μm). Free amino acids and their metabolites were measured in the spent culture medium from each group. In cultured ovarian cortical strips, the concentration of α-aminoadipic acid was significantly higher in the low activation group than in the high group ( < 0.05), while those of methionine, lysine, and arginine were higher in the high activation group. In cultured isolated secondary follicles, concentrations of methionine, tyrosine, histidine, and hydroxyproline were higher in the degenerate group ( ≤ 0.05). In conclusion, amino acid metabolism has the potential to serve as an indicator of primordial follicle activation and subsequent growth rate during bovine IVG.
为了确定体外生长（IVG）过程中牛卵泡健康状况的潜在指标，对牛卵泡的氨基酸代谢进行了评估。将牛卵巢皮质切片，制成条带，并培养6天。在培养6天前后对组织样本进行组织学检查，并根据生长中的次级卵泡数量将卵泡激活程度分为高或低（高：711个；低：01个）。在另一个实验中，手动分离并培养次级卵泡（直径范围：100200μm），并监测其生长6天。根据培养过程中的直径变化将培养的卵泡分为生长型或退化型（生长型：直径增加60.574.1μm；退化型：直径减少28~15.2μm）。测量每组废弃培养基中的游离氨基酸及其代谢产物。在培养的卵巢皮质条带中，低激活组中α-氨基己二酸的浓度显著高于高激活组（<0.05），而高激活组中甲硫氨酸、赖氨酸和精氨酸的浓度较高。在培养的分离次级卵泡中，退化组中甲硫氨酸、酪氨酸、组氨酸和羟脯氨酸的浓度较高（≤0.05）。总之，氨基酸代谢有可能作为牛IVG过程中原始卵泡激活及后续生长速度的指标。
