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亚洲蓝升麻中小檗碱合成途径相关基因的分离与鉴定

Isolation and Characterization of the Genes Involved in the Berberine Synthesis Pathway in Asian Blue Cohosh, .

作者信息

Park Nam-Il, Roy Neha Samir, Park Yeri, Choi Beom-Soon, Jeon Mi Jin, Oh Ji Yeon, Kim Bo-Yun, Kim Young-Dong, Kim Yong-In, Um Taeyoung, Kwak Hwan Jong, Kim Nam-Soo, Kim Soonok, Choi Ik-Young

机构信息

Department of Plant Science, Gangneung-Wonju National University, Gangneung 25457, Republic of Korea.

Agriculture and Life Sciences Research Institute, Kangwon National University, Chuncheon 24341, Republic of Korea.

出版信息

Plants (Basel). 2023 Mar 28;12(7):1483. doi: 10.3390/plants12071483.

DOI:10.3390/plants12071483
PMID:37050109
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10096549/
Abstract

, commonly named Asian blue cohosh, is a perennial herb in the family Berberidaceae. It has traditionally been used for folk medicine in China. We isolated berberine from the leaves, stem, roots, and fruits of , and this is the first report on berberine in this species. Transcriptome analysis was conducted for the characterization of berberine biosynthesis genes in , in which, all the genes for berberine biosynthesis were identified. From 40,094 transcripts, using gene ontology (GO) analysis, 26,750 transcripts were assigned their functions in the categories of biological process, molecular function, and cellular component. In the analysis of genes expressed in different tissues, the numbers of genes in the categories of intrinsic component of membrane and transferase activity were up-regulated in leaves versus stem. The berberine synthesis genes in were characterized by phylogenetic analysis with corresponding genes from other berberine-producing species. The co-existence of genes from different plant families in the deepest branch subclade implies that the differentiation of berberine synthesis genes occurred early in the evolution of berberine-producing plants. Furthermore, the copy number increment of the berberine synthesis genes was detected at the species level.

摘要

，通常被称为亚洲蓝升麻，是小檗科的一种多年生草本植物。它在中国传统上被用于民间医药。我们从该植物的叶、茎、根和果实中分离出了黄连素，这是关于该物种中黄连素的首次报道。对该植物进行了转录组分析以表征黄连素生物合成基因，其中鉴定出了所有黄连素生物合成基因。从40,094个转录本中，通过基因本体（GO）分析，有26,750个转录本在生物过程、分子功能和细胞成分类别中被赋予了功能。在不同组织中表达的基因分析中，与茎相比，叶中膜内在成分和转移酶活性类别中的基因数量上调。通过与其他产黄连素物种的相应基因进行系统发育分析，对该植物中的黄连素合成基因进行了表征。来自不同植物科的基因在最深分支亚分支中的共存意味着黄连素合成基因的分化发生在产黄连素植物进化的早期。此外，在物种水平上检测到了黄连素合成基因的拷贝数增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/e46874d5eb70/plants-12-01483-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/7618b3b9f80f/plants-12-01483-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/80a8343443dd/plants-12-01483-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/e5a33900547e/plants-12-01483-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/729f5ef4c177/plants-12-01483-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/7e406ae800f6/plants-12-01483-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/6726fd4477aa/plants-12-01483-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/7b56e38381e5/plants-12-01483-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/e46874d5eb70/plants-12-01483-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/7618b3b9f80f/plants-12-01483-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/80a8343443dd/plants-12-01483-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/e5a33900547e/plants-12-01483-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/729f5ef4c177/plants-12-01483-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/7e406ae800f6/plants-12-01483-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/6726fd4477aa/plants-12-01483-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/7b56e38381e5/plants-12-01483-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d7b/10096549/e46874d5eb70/plants-12-01483-g008.jpg

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