阳离子和某些化合物对百草枯在培养肺细胞中蓄积的影响。

Effect of cations and some compounds on paraquat accumulation into cultured pneumocytes.

作者信息

Saito K

出版信息

Tohoku J Exp Med. 1986 Jan;148(1):41-7. doi: 10.1620/tjem.148.41.

DOI:10.1620/tjem.148.41

Abstract

Paraquat and diquat, bipyridilium herbicides, have been shown to be accumulated into pneumocytes of three species in culture. Paraquat at 10(-3) M was accumulated to a maximum of 3-7 nmol/mg protein for 20 min. Diquat accumulated more slowly but reached a concentration of 1-6 nmol/mg protein for 60 min. Differences in the ability of various pneumocytes to accumulate paraquat and diquat appear to exist. It was found that the cations, Ca++, Na+ and K+, reduced the accumulation of paraquat. Valinomycin stimulated paraquat accumulation significantly. On the other hand, paraquat accumulation was inhibited by 2, 4-dinitrophenol. These results indicate that paraquat may be accumulated, in part, as cation species into pneumocytes and also by a energy-dependent process regulated by cellular ATP.

摘要

百草枯和敌草快这两种联吡啶类除草剂已被证明可在培养的三种细胞的肺细胞中蓄积。10⁻³ M的百草枯在20分钟内蓄积量最高可达3 - 7 nmol/mg蛋白质。敌草快蓄积得较慢，但在60分钟时达到1 - 6 nmol/mg蛋白质的浓度。不同肺细胞蓄积百草枯和敌草快的能力似乎存在差异。研究发现，阳离子Ca²⁺、Na⁺和K⁺会降低百草枯的蓄积。缬氨霉素显著刺激百草枯的蓄积。另一方面，2,4 -二硝基苯酚抑制百草枯的蓄积。这些结果表明，百草枯可能部分以阳离子形式蓄积到肺细胞中，并且也是通过由细胞ATP调节的能量依赖过程。