Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Insect Molecular Genetics and Biotechnology, Institute of Biosciences and Applications, National Centre for Scientific Research Demokritos, Aghia Paraskevi, Athens 15341, Greece.
J Agric Food Chem. 2023 Apr 26;71(16):6338-6347. doi: 10.1021/acs.jafc.3c00927. Epub 2023 Apr 13.
cypovirus 1 (BmCPV1), a primary pathogen of the silkworm, is a typical dsRNA virus belonging to the family. In this study, a total of 2520 differentially expressed genes (DEGs) were identified by RNA-seq analysis of the silkworm midgut after BmCPV1 infection and Gene Ontology (GO) functional annotation showed that the DEGs predominantly functioned in binding (molecular function), cell (cellular component), and cellular processes (biological process). Additionally, the Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotation revealed that the DEGs were mainly distributed in global and overview metabolism maps, translation, and signal transduction. Among the identified DEGs, belongs to the peptidoglycan recognition protein (PGRP) family. Previous studies have revealed that PGRPs were involved in the interactions between silkworm and BmCPV1. Here, we explored the effect of BmPGRP-S5 on BmCPV1 replication and demonstrated that BmPGRP-S5 promotes the proliferation of BmCPV1 in BmN cells through overexpression or knockdown experiments. Knocking down of in silkworm larvae similarly promoted the proliferation of BmCPV1. Through experimental validation, we therefore determined that BmPGRP-S5 acts as a proviral host factor for BmCPV1 infection. This study clarifies the proliferation mechanism of BmCPV1 and provides new insights into the functional role of BmPGRP-S5.
杆状病毒 1（BmCPV1）是家蚕的主要病原体，是一种典型的双链 RNA 病毒，属于 科。在本研究中，通过 RNA-seq 分析感染 BmCPV1 后家蚕中肠的转录组数据，共鉴定出 2520 个差异表达基因（DEGs）。GO 功能注释显示，这些 DEGs 主要参与结合（分子功能）、细胞（细胞成分）和细胞过程（生物过程）。此外，京都基因与基因组百科全书（KEGG）功能注释表明，DEGs 主要分布在全局和概览代谢图谱、翻译和信号转导中。在所鉴定的 DEGs 中， 属于肽聚糖识别蛋白（PGRP）家族。先前的研究表明，PGRPs 参与了家蚕与 BmCPV1 之间的相互作用。在这里，我们研究了 BmPGRP-S5 对 BmCPV1 复制的影响，并通过过表达或敲低实验证明，BmPGRP-S5 通过促进 BmCPV1 在 BmN 细胞中的增殖来促进 BmCPV1 的增殖。在家蚕幼虫中敲低 同样促进了 BmCPV1 的增殖。通过实验验证，我们确定 BmPGRP-S5 是 BmCPV1 感染的辅助病毒宿主因子。本研究阐明了 BmCPV1 的增殖机制，并为 BmPGRP-S5 的功能作用提供了新的见解。
