Zhang Yinong, Yan Jiming, Xie Yukai, Wang Xiong, Ren Feifei, Bian Haixu, Sun Jingchen
Subtropical Sericulture and Mulberry Resources Protection and Safety Engineering Research Center, Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Insects. 2025 Apr 19;16(4):431. doi: 10.3390/insects16040431.
Insects detect pathogens through their germ-line encoded pattern recognition receptors (PRRs). Among these, β-1,3-glucan recognition protein (βGRP) is a crucial PRR that specifically identifies pathogenic microorganisms and triggers innate immune signaling cascades. However, it remains unclear whether βGRP can detect viruses and protect the host from viral threats. In this study, using high-throughput sequencing technology, we observed a significant suppression of βGRP-3 in during infection with the cytoplasmic polyhedrosis virus (BmCPV). Moreover, overexpression of βGRP-3 in BmN cell lines resulted in a reduction of BmCPV proliferation, whereas knockdown of βGRP-3 in BmN cells promoted BmCPV proliferation. These findings suggest that the βGRP family functions not only as anti-bacterial, antifungal, and anti-yeast PRRs but also as protectors against various harmful viruses in insects.
昆虫通过其种系编码的模式识别受体（PRR）来检测病原体。其中，β-1,3-葡聚糖识别蛋白（βGRP）是一种关键的PRR，它能特异性识别病原微生物并触发先天性免疫信号级联反应。然而，βGRP是否能检测病毒并保护宿主免受病毒威胁仍不清楚。在本研究中，我们使用高通量测序技术观察到，在感染细胞质多角体病毒（BmCPV）期间，βGRP-3显著受到抑制。此外，在BmN细胞系中过表达βGRP-3导致BmCPV增殖减少，而在BmN细胞中敲低βGRP-3则促进BmCPV增殖。这些发现表明，βGRP家族不仅作为抗细菌、抗真菌和抗酵母的PRR发挥作用，还作为昆虫抵御各种有害病毒的保护者发挥作用。
