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甘蓝型油菜 NsaCMS 和 PolCMS 败育特征的比较研究及与花粉败育相关的长非编码 RNA 分析。

Comparative study on abortion characteristics of Nsa CMS and Pol CMS and analysis of long non-coding RNAs related to pollen abortion in Brassica napus.

机构信息

Hunan Branch of National Oilseed Crops Improvement Center, Changsha, China.

College of Agriculture, Hunan Agricultural University, Changsha, China.

出版信息

PLoS One. 2023 Apr 13;18(4):e0284287. doi: 10.1371/journal.pone.0284287. eCollection 2023.

DOI:10.1371/journal.pone.0284287
PMID:37053132
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10101420/
Abstract

Cytoplasmic male sterile system (CMS) is one of the important methods for the utilization of heterosisin Brassica napus. The involvement of long non-coding RNAs (lncRNAs) in anther and pollen development in B.napus has been recognized, but there is little data on the involvement of lncRNAs in pollen abortion in different types of rapeseed CMS. The present study compared the cytological, physiological and biochemical characteristics of Nsa CMS (1258A) and Pol CMS (P5A) during pollen abortion, and high-throughput sequencing of flower buds of different sizes before and after pollen abortion. The results showed that insufficient energy supply was an important physiological basis for 1258A and P5A pollen abortion, and 1258A had excessive ROS (reactive oxygen species) accumulation in the stage of pollen abortion. Functional analysis showed that Starch and sucrose metabolism and Sulfur metabolism were significantly enriched before and after pollen abortion in 1258A and P5A, and a large number of genes were down-regulated. In 1258A, 227 lncRNAs had cis-targeting regulation, and 240 cis-target genes of the lncRNAs were identified. In P5A, 116 lncRNAs had cis-targeting regulation, and 101 cis-target genes of the lncRNAs were identified. There were five lncRNAs cis-target genes in 1258A and P5A during pollen abortion, and LOC106445716 encodes β-D-glucopyranosyl abscisate β-glucosidase and could regulate pollen abortion. Taken together, this study, provides a new perspective for lncRNAs to participate in the regulation of Nsa CMS and Pol CMS pollen abortion.

摘要

细胞质雄性不育（CMS）系统是甘蓝型油菜杂种优势利用的重要方法之一。长链非编码 RNA（lncRNA）参与油菜花药和花粉发育已得到公认，但关于 lncRNA 参与不同类型油菜 CMS 花粉败育的研究较少。本研究比较了 Nsa CMS（1258A）和 Pol CMS（P5A）在花粉败育过程中的细胞学、生理学和生物化学特征，以及花粉败育前后不同大小花蕾的高通量测序。结果表明，能量供应不足是 1258A 和 P5A 花粉败育的重要生理基础，1258A 在花粉败育阶段 ROS（活性氧）积累过多。功能分析表明，淀粉和蔗糖代谢以及硫代谢在 1258A 和 P5A 花粉败育前后均显著富集，大量基因下调。在 1258A 中，有 227 个 lncRNA 具有顺式靶向调控，鉴定到 lncRNA 的 240 个顺式靶基因。在 P5A 中，有 116 个 lncRNA 具有顺式靶向调控，鉴定到 lncRNA 的 101 个顺式靶基因。在 1258A 和 P5A 花粉败育过程中，有 5 个 lncRNA 顺式靶基因，LOC106445716 编码 β-D-葡萄糖基脱落酸 β-葡萄糖苷酶，可调控花粉败育。综上所述，本研究为 lncRNA 参与 Nsa CMS 和 Pol CMS 花粉败育调控提供了新视角。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/db0fafed98dd/pone.0284287.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/9e4ce30171fd/pone.0284287.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/f5c53b8207bf/pone.0284287.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/cff74c5c20d3/pone.0284287.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/ef1a99cf0585/pone.0284287.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/3eb782ff1974/pone.0284287.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/c8f2b1ccc890/pone.0284287.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/c4a52a5d04c4/pone.0284287.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/fec6556ad87c/pone.0284287.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/db0fafed98dd/pone.0284287.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/9e4ce30171fd/pone.0284287.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/f5c53b8207bf/pone.0284287.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/cff74c5c20d3/pone.0284287.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/ef1a99cf0585/pone.0284287.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/3eb782ff1974/pone.0284287.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/c8f2b1ccc890/pone.0284287.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/c4a52a5d04c4/pone.0284287.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/fec6556ad87c/pone.0284287.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f1/10101420/db0fafed98dd/pone.0284287.g009.jpg

相似文献

[1]
Comparative study on abortion characteristics of Nsa CMS and Pol CMS and analysis of long non-coding RNAs related to pollen abortion in Brassica napus.

PLoS One. 2023

[2]
Comparative Cytological and Transcriptome Analyses of Anther Development in Cytoplasmic Male Sterile (1258A) and Maintainer Lines in Produced by Distant Hybridization.

Int J Mol Sci. 2022-2-11

[3]
Transcriptome and Hormone Comparison of Three Cytoplasmic Male Sterile Systems in .

Int J Mol Sci. 2018-12-12

[4]
Genome-wide analysis of mRNA and lncRNA expression and mitochondrial genome sequencing provide insights into the mechanisms underlying a novel cytoplasmic male sterility system, BVRC-CMS96, in Brassicarapa.

Theor Appl Genet. 2020-7

[5]
Comparative transcript profiling of the fertile and sterile flower buds of pol CMS in B. napus.

BMC Genomics. 2014-4-3

[6]
Comparative Transcriptome Analysis between Fertile and CMS Flower Buds in Wucai (Brassica campestris L.).

BMC Genomics. 2018-12-12

[7]
Molecular Analysis Uncovers the Mechanism of Fertility Restoration in Temperature-Sensitive Polima Cytoplasmic Male-Sterile .

Int J Mol Sci. 2021-11-18

[8]
Microarray analysis reveals altered expression of a large number of nuclear genes in developing cytoplasmic male sterile Brassica napus flowers.

Plant J. 2007-2

[9]
Abnormal tapetum development and energy metabolism associated with sterility in SaNa-1A CMS of Brassica napus L.

Plant Cell Rep. 2019-1-31

[10]
Comparative transcriptome analysis provides insight into the important pathways and key genes related to the pollen abortion in the thermo-sensitive genic male sterile line 373S in Brassica napus L.

Funct Integr Genomics. 2022-12-28

本文引用的文献

[1]
Comparative Cytological and Transcriptome Analyses of Anther Development in Cytoplasmic Male Sterile (1258A) and Maintainer Lines in Produced by Distant Hybridization.

Int J Mol Sci. 2022-2-11

[2]
Transcript Profiling Analysis and ncRNAs' Identification of Male-Sterile Systems of Reveal New Insights Into the Mechanism Underlying Anther and Pollen Development.

Front Plant Sci. 2022-2-8

[3]
Circular RNAs Repertoire and Expression Profile during Pollen Development.

Int J Mol Sci. 2021-9-24

[4]
Global identification of long non-coding RNAs involved in the induction of spinach flowering.

BMC Genomics. 2021-9-30

[5]
Interplay between miRNAs and lncRNAs: Mode of action and biological roles in plant development and stress adaptation.

Comput Struct Biotechnol J. 2021-4-27

[6]
Long Noncoding RNAs in Plants.

Annu Rev Plant Biol. 2021-6-17

[7]
Os4BGlu14, a monolignol β-Glucosidase, negatively affects seed longevity by influencing primary metabolism in rice.

Plant Mol Biol. 2020-8-24

[8]
Activation of Mitochondrial orf355 Gene Expression by a Nuclear-Encoded DREB Transcription Factor Causes Cytoplasmic Male Sterility in Maize.

Mol Plant. 2020-9-7

[9]
Uncloaking lncRNA-meditated gene expression as a potential regulator of CMS in cotton (Gossypium hirsutum L.).

Genomics. 2020-9

[10]
Integrative Analysis of the lncRNA and mRNA Transcriptome Revealed Genes and Pathways Potentially Involved in the Anther Abortion of Cotton ( L.).

Genes (Basel). 2019-11-20

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