Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, China.
Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, China.
Pathol Res Pract. 2023 May;245:154426. doi: 10.1016/j.prp.2023.154426. Epub 2023 Mar 27.
To investigate the expression of chromosome 6 open reading frame 15 (C6orf15) in colon cancer and its effects on clinicopathological features and prognosis.
Using the transcriptome and clinical data of colon cancer and normal tissues in The Cancer Genome Atlas (TCGA) database, the expression of C6orf15 mRNA in colon cancer samples and its relationship with clinicopathological characteristics and prognosis were explored. The expression level of C6orf15 protein in 23 colon cancer tissues was detected by immunohistochemistry (IHC). The possible mechanism of C6orf15 involved in the occurrence and development of colon cancer was explored by gene set enrichment analysis (GSEA).
Compared with normal tissues, C6orf15 was highly expressed in colon cancer (1.207 ± 0.694 vs 0.276 ± 0.166, t = 8.281, P < 0.01). The expression level of C6orf15 was associated with tumor invasion depth (χ2 = 8.30, P = 0.04), lymph node metastasis (χ2 = 36.97, P < 0.001), distant metastasis (χ2 = 8.69, P = 0.003) and pathological stage (χ2 = 34.17, P < 0.001). High expression of C6orf15 was associated with poor prognosis (χ2 = 6.43, P < 0.05). The results of GSEA showed that C6orf15 promotes the occurrence and development of colon cancer by promoting the ECM receptor interaction pathway, Hedgehog signaling pathway and Wnt signaling pathway. Immunohistochemical results showed that the expression of C6orf15 protein in colon cancer tissues was correlated with the depth of invasion (P = 0.023) and lymph node metastasis (P = 0.048).
C6orf15 is highly expressed in colon cancer tissue and is related to adverse pathological features and poor prognosis of colon cancer. It is involved in multiple oncogenic signaling pathways and may serve as a prognostic marker of colon cancer.
研究 6 号染色体开放阅读框 15（C6orf15）在结肠癌中的表达及其对临床病理特征和预后的影响。
利用癌症基因组图谱（TCGA）数据库中结肠癌及正常组织的转录组和临床数据，探索 C6orf15mRNA 在结肠癌样本中的表达及其与临床病理特征和预后的关系。采用免疫组织化学（IHC）检测 23 例结肠癌组织中 C6orf15 蛋白的表达。通过基因集富集分析（GSEA）探讨 C6orf15 参与结肠癌发生发展的可能机制。
与正常组织相比，结肠癌中 C6orf15 表达较高（1.207±0.694 比 0.276±0.166，t=8.281，P<0.01）。C6orf15 的表达水平与肿瘤浸润深度（χ2=8.30，P=0.04）、淋巴结转移（χ2=36.97，P<0.001）、远处转移（χ2=8.69，P=0.003）和病理分期（χ2=34.17，P<0.001）相关。C6orf15 高表达与预后不良相关（χ2=6.43，P<0.05）。GSEA 结果表明，C6orf15 通过促进 ECM 受体相互作用通路、Hedgehog 信号通路和 Wnt 信号通路促进结肠癌的发生和发展。免疫组化结果显示，结肠癌组织中 C6orf15 蛋白的表达与浸润深度（P=0.023）和淋巴结转移（P=0.048）相关。
C6orf15 在结肠癌组织中高表达，与结肠癌不良的病理特征和预后相关。它参与多个致癌信号通路，可能成为结肠癌的预后标志物。
