[不同条件对耻垢分枝杆菌丁酸细胞中DNA甲基化酶稳定性的影响]
[Effect of various conditions on the stability of DNA-methylases from Mycobacterium smegmatis butyricum cells].
作者信息
Sharkova E V, Nikol'skaia I I, Shomodi P, Fel'desh I, Debov S S
出版信息
Vopr Med Khim. 1986 Mar-Apr;32(2):125-9.
DNA-methylases from M. sm. butyricum, produced by means of isoelectrofocusing, were characterized by slow spontaneous variations in the activity with amplitude of 75-80% under conditions of storage in glycerol at -10 degrees. Effect of various conditions on stabilization of activity of adenine methylases MMbu4,2 and MMbu7,2 was studied. Alterations in the enzymatic activity were not found in presence of substances applied usually to stabilize the enzymes, blood serum albumin and cations of two valent metals as the values of the alterations occurred in the ranges of the enzyme activity variations.
通过等电聚焦制备的丁酸梭菌DNA甲基化酶，在-10℃甘油储存条件下，其活性呈现缓慢的自发变化，变化幅度为75-80%。研究了各种条件对腺嘌呤甲基化酶MMbu4,2和MMbu7,2活性稳定性的影响。在通常用于稳定酶的物质、血清白蛋白和二价金属阳离子存在的情况下，未发现酶活性有变化，因为这些变化值发生在酶活性变化范围内。
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