Izatullaev E A, Talbaev T D, Mamirova T N, Urbisinov Zh K
Vopr Pitan. 1986 Mar-Apr(2):64-7.
The authors describe methods for isolation and assay of free amino acids in body tissues employed today in clinical and experimental studies. Give an account of the main factors affecting the data obtained. Compare the efficacy of different methods for quantitative determination of free amino acids in tissues. Analyse difficulties arising in the option of the deproteinizing agent while preparing tissue samples to be tested. Based on the reported data and their own experience the authors suggest efficacious and less time-consuming research methods for assay of free amino acids in blood plasma, red blood cells, and tissue homogenates.
作者描述了目前临床和实验研究中用于分离和测定人体组织中游离氨基酸的方法。阐述影响所获数据的主要因素。比较不同方法定量测定组织中游离氨基酸的效能。分析在制备待测组织样本时选择脱蛋白剂所产生的困难。基于所报道的数据及自身经验，作者提出了测定血浆、红细胞和组织匀浆中游离氨基酸的高效且耗时较短的研究方法。
