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一种来自哥斯达黎加油井的新型种间缔合群落，由 Methanobacterium cahuitense sp. nov. 和 Desulfomicrobium aggregans sp. nov. 组成。

A novel interdomain consortium from a Costa Rican oil well composed of Methanobacterium cahuitense sp. nov. and Desulfomicrobium aggregans sp. nov.

机构信息

Institute of Microbiology and Archaea Centre, University of Regensburg, Universitätsstrasse 31, 93053, Regensburg, Germany.

Plant Development and Electron Microscopy, Biocenter LMU Munich, Planegg-Martinsried, Germany.

出版信息

Arch Microbiol. 2023 Apr 13;205(5):189. doi: 10.1007/s00203-023-03533-9.

DOI:10.1007/s00203-023-03533-9
PMID:37055657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10102059/
Abstract

A novel interdomain consortium composed of a methanogenic Archaeon and a sulfate-reducing bacterium was isolated from a microbial biofilm in an oil well in Cahuita National Park, Costa Rica. Both organisms can be grown in pure culture or as stable co-culture. The methanogenic cells were non-motile rods producing CH exclusively from H/CO. Cells of the sulfate-reducing partner were motile rods forming cell aggregates. They utilized hydrogen, lactate, formate, and pyruvate as electron donors. Electron acceptors were sulfate, thiosulfate, and sulfite. 16S rRNA sequencing revealed 99% gene sequence similarity of strain CaP3V-M-L2A to Methanobacterium subterraneum and 98.5% of strain CaP3V-S-L1A to Desulfomicrobium baculatum. Both strains grew from 20 to 42 °C, pH 5.0-7.5, and 0-4% NaCl. Based on our data, type strains CaP3V-M-L2A (= DSM 113354 = JCM 39174) and CaP3V-S-L1A (= DSM 113299 = JCM 39179) represent novel species which we name Methanobacterium cahuitense sp. nov. and Desulfomicrobium aggregans sp. nov.

摘要

从哥斯达黎加卡胡塔国家公园一口油井中的微生物生物膜中分离出一种新型的域间联合体，由产甲烷古菌和硫酸盐还原菌组成。这两种生物都可以在纯培养或稳定共培养中生长。产甲烷细胞为非运动杆状细胞，仅能从 H/CO 中产生 CH。硫酸盐还原伙伴的细胞为运动杆状细胞，形成细胞聚集物。它们利用氢气、乳酸盐、甲酸盐和丙酮酸作为电子供体。电子受体是硫酸盐、硫代硫酸盐和亚硫酸盐。16S rRNA 测序显示，菌株 CaP3V-M-L2A 与 Methanobacterium subterraneum 的基因序列相似度为 99%，菌株 CaP3V-S-L1A 与 Desulfomicrobium baculatum 的相似度为 98.5%。两种菌株均能在 20-42°C、pH5.0-7.5 和 0-4%NaCl 的条件下生长。根据我们的数据，模式菌株 CaP3V-M-L2A（=DSM 113354=JCM 39174）和 CaP3V-S-L1A（=DSM 113299=JCM 39179）代表了新型物种，我们将其命名为 Methanobacterium cahuitense sp. nov. 和 Desulfomicrobium aggregans sp. nov.

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d83/10102059/ab6c7533ae2d/203_2023_3533_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d83/10102059/0c022b5ee3c0/203_2023_3533_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d83/10102059/9e2207abca52/203_2023_3533_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d83/10102059/5c89f09ba2b3/203_2023_3533_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d83/10102059/ab6c7533ae2d/203_2023_3533_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d83/10102059/0c022b5ee3c0/203_2023_3533_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d83/10102059/9e2207abca52/203_2023_3533_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d83/10102059/5c89f09ba2b3/203_2023_3533_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d83/10102059/ab6c7533ae2d/203_2023_3533_Fig4_HTML.jpg

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引用本文的文献

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本文引用的文献

[1]
Methanofollis propanolicus sp. nov., a novel archaeal isolate from a Costa Rican oil well that uses propanol for methane production.

Arch Microbiol. 2022-8-13

[2]
Microbially induced corrosion impacts on the oil industry.

Arch Microbiol. 2022-1-15

[3]
The hydrogen threshold of obligately methyl-reducing methanogens.

FEMS Microbiol Lett. 2020-9-1

[4]
Assembly of long, error-prone reads using repeat graphs.

Nat Biotechnol. 2019-4-1

[5]
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.

Mol Biol Evol. 2018-6-1

[6]
Why do sulfate-reducing bacteria outcompete methanogenic bacteria for substrates?

Oecologia. 1983-11

[7]
The temperature gradient-forming device, an accessory unit for normal light microscopes to study the biology of hyperthermophilic microorganisms.

Appl Environ Microbiol. 2014-8

[8]
MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.

Mol Biol Evol. 2013-10-16

[9]
Structural foundations for the O2 resistance of Desulfomicrobium baculatum [NiFeSe]-hydrogenase.

Chem Commun (Camb). 2013-8-14

[10]
FastTree 2--approximately maximum-likelihood trees for large alignments.

PLoS One. 2010-3-10

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