Institute of Microbiology and Archaea Centre, University of Regensburg, Universitaetsstrasse 31, 93053, Regensburg, Germany.
Electron Microscopy Center, University of Regensburg, Universitaetsstrasse 31, 93053, Regensburg, Germany.
Arch Microbiol. 2022 Aug 13;204(9):554. doi: 10.1007/s00203-022-03152-w.
A novel methanogenic strain, CaP3V-MF-L2A, was isolated from an exploratory oil well from Cahuita National Park, Costa Rica. The cells were irregular cocci, 0.8-1.8 μm in diameter, stained Gram-negative and were motile. The strain utilized H/CO, formate and the primary and secondary alcohols 1-propanol and 2-propanol for methanogenesis, but not acetate, methanol, ethanol, 1-butanol or 2-butanol. Acetate was required as carbon source. The novel isolate grew at 25-40 °C, pH 6.0-7.5 and 0-2.5% (w/v) NaCl. 16S rRNA gene sequence analysis revealed that the strain is affiliated to the genus Methanofollis. It shows 98.8% sequence similarity to its closest relative Methanofollis ethanolicus. The G + C content is 60.1 mol%. Based on the data presented here type strain CaP3V-MF-L2A (= DSM 113321 = JCM 39176) represents a novel species, Methanofollis propanolicus sp. nov.
从哥斯达黎加卡胡塔国家公园的一口勘探油井中分离到一株产甲烷新菌株 CaP3V-MF-L2A。细胞呈不规则球菌，直径 0.8-1.8μm，革兰氏染色阴性，可运动。该菌株利用 H/CO、甲酸盐以及一级和二级醇 1-丙醇和 2-丙醇进行产甲烷作用，但不利用乙酸盐、甲醇、乙醇、1-丁醇或 2-丁醇。乙酸盐是必需的碳源。新型分离株在 25-40°C、pH6.0-7.5 和 0-2.5%（w/v）NaCl 下生长。16S rRNA 基因序列分析表明，该菌株属于 Methanofollis 属。它与最接近的亲缘关系 Methanofollis ethanolicus 具有 98.8%的序列相似性。G+C 含量为 60.1mol%。基于这里提出的数据，模式菌株 CaP3V-MF-L2A（=DSM 113321=JCM 39176）代表了一个新的物种，即 Methanofollis propanolicus sp. nov.
