Korman S H, Le Blancq S M, Spira D T, el On J, Reifen R M, Deckelbaum R J
Z Parasitenkd. 1986;72(2):173-80. doi: 10.1007/BF00931144.
Four axenically cultured human Giardia lamblia isolates from Jerusalem (KC-1, 2, 3 and 4) and one from Bethesda (WB) were compared. Three distinct groups were defined by agglutination response to rabbit anti-G. lamblia sera viz. WB; KC-3; and KC-1, 2 and 4. The same major groups were identified by isoenzyme analysis using thin-layer starch-gel electrophoresis, each group differing from the others in three or more of five enzymes studied. In addition, a single enzyme difference distinguished KC-2 from KC-1 and 4. These findings reveal significant heterogeneity in G. lamblia isolates both from widely separated areas and within a single region. Immunoassays for diagnosis of giardiasis should take into account the differences between strains. Heterogeneity among G. lamblia strains may explain the variable clinical manifestations, host response and treatment efficacy characteristic of human giardiasis.
对来自耶路撒冷的4株经无菌培养的人源蓝氏贾第鞭毛虫分离株（KC - 1、2、3和4）和1株来自贝塞斯达的分离株（WB）进行了比较。根据对兔抗蓝氏贾第鞭毛虫血清的凝集反应确定了3个不同的组，即WB组；KC - 3组；以及KC - 1、2和4组。通过使用薄层淀粉凝胶电泳的同工酶分析确定了相同的主要组，在研究的5种酶中，每组在3种或更多种酶上与其他组不同。此外，一种酶的差异将KC - 2与KC - 1和4区分开来。这些发现揭示了来自广泛分离地区以及单个区域内的蓝氏贾第鞭毛虫分离株存在显著的异质性。用于贾第虫病诊断的免疫测定应考虑菌株之间的差异。蓝氏贾第鞭毛虫菌株之间的异质性可能解释了人类贾第虫病的可变临床表现、宿主反应和治疗效果。
