对苯乙醛的气味受体的反应及对稻水象甲（）行为的影响。

Response of Odorant Receptors with Phenylacetaldehyde and the Effects on the Behavior of the Rice Water Weevil ().

机构信息

College of Plant Science, Jilin University, Changchun 130062, PR China.

College of Plant Protection, Northeast Agricultural University, Harbin 150030, PR China.

出版信息

J Agric Food Chem. 2023 May 3;71(17):6541-6551. doi: 10.1021/acs.jafc.2c07963. Epub 2023 Apr 14.

DOI:10.1021/acs.jafc.2c07963

PMID:37058441

Abstract

The rice water weevil (RWW), Kuschel (Coleoptera: Curculionidae), is a destructive rice pest that threatens the rice industry worldwide. Odorant receptors (ORs) and odorant receptor coreceptors (Orcos) play an important role in the process of insects' whole life activities; however, there are no related functional studies on RWW. On this basis, a heterologous study of LoryOR20/LoryOrco in oocytes was performed to detect the effects of certain natural compounds on RWWs and four active compounds were found. Electroantennogram (EAG) recordings and a behavior test showed that RWWs exhibited a significant response to phenylacetaldehyde (PAA) and an EAG measurement of dsRNA-LoryOR20-treated RWWs revealed a significant decrease in response to PAA. Our results revealed an olfactory molecular mechanism for the recognition of PAA by RWWs, thus providing a potential genetic target at the peripheral olfactory sensing level, contributing to the development of novel control strategies for pest management.

摘要

稻水象甲（RWW），Kuschel（鞘翅目：象甲科），是一种破坏性的水稻害虫，威胁着全球的水稻产业。气味受体（ORs）和气味受体共受体（Orcos）在昆虫的整个生命活动过程中起着重要的作用；然而，在 RWW 中还没有相关的功能研究。在此基础上，在卵母细胞中对 LoryOR20/LoryOrco 进行了异源研究，以检测某些天然化合物对 RWW 的影响，发现了四种活性化合物。触角电位（EAG）记录和行为测试表明，RWW 对苯乙醛（PAA）表现出显著的反应，而 EAG 测量表明 dsRNA-LoryOR20 处理的 RWW 对 PAA 的反应显著降低。我们的研究结果揭示了 RWW 识别 PAA 的嗅觉分子机制，从而为周边嗅觉感应水平的害虫管理提供了潜在的遗传靶标，有助于开发新的控制策略。