大鼠血液和脑脊液中α-促黑素细胞激素样免疫反应性的特征

Characterization of the alpha-MSH-like immunoreactivity in blood and cerebrospinal fluid of the rat.

作者信息

De Rotte A A, Verhoef J, Andringa-Bakker E A, Van Wimersma Greidanus T B

出版信息

Acta Endocrinol (Copenh). 1986 Apr;111(4):440-4. doi: 10.1530/acta.0.1110440.

DOI:10.1530/acta.0.1110440

Abstract

We have investigated the nature of the alpha-melanocyte stimulating hormone-like immunoreactivity (alpha-MSH-LI) in blood and cerebrospinal fluid (CSF) of the rat. Blood and CSF from intact animals were subjected to high pressure liquid chromatography (HPLC) followed by a radioimmunoassay (RIA) specific for the C-terminal part of the alpha-MSH molecule. It appeared that in both body fluids the predominant alpha-MSH-LI co-migrated with synthetic alpha-MSH and not with its des-acetyl or di-acetyl analogues. We conclude that alpha-MSH is the predominant form of alpha-MSH-LI circulating in plasma and CSF of rats from our Wistar strain.

摘要

我们研究了大鼠血液和脑脊液（CSF）中α-黑素细胞刺激素样免疫反应性（α-MSH-LI）的性质。对完整动物的血液和脑脊液进行高压液相色谱（HPLC），然后进行针对α-MSH分子C末端部分的放射免疫分析（RIA）。结果显示，在两种体液中，主要的α-MSH-LI与合成的α-MSH共迁移，而不与其去乙酰或二乙酰类似物共迁移。我们得出结论，α-MSH是来自我们Wistar品系大鼠血浆和脑脊液中循环的α-MSH-LI的主要形式。