Laboratory of ecological immunology of aquatic organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Leninsky ave, 38, Moscow 119991, Russia.
Laboratory of ecological immunology of aquatic organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Leninsky ave, 38, Moscow 119991, Russia; Department of Radiation and Chemical Biology, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Leninsky ave, 38, Moscow 119991, Russia.
Mar Pollut Bull. 2023 Jun;191:114923. doi: 10.1016/j.marpolbul.2023.114923. Epub 2023 Apr 13.
Mussels were exposed to di-(2-ethylhexyl) phthalate (DEHP) (0.4 mg L and 4.0 mg L) for 24 h and 48 h and its effect on hemocyte cellular composition and spontaneous reactive oxygen production (ROS) levels in hemocytes were evaluated. Exposure to DEHP induced a loss in spontaneous ROS production levels in hemocytes and decreased agranulocyte number in hemolymph. DEHP was found to accumulate in hepatopancreas of mussels and this process was associated with an increase of catalase (CAT) activity after 24 h incubation. At the end of the experimental period (48 h) CAT activity recovered up to control levels. Superoxide dismutase (SOD) activity in hepatopancreas increased following 48 h exposure to DEHP. The results indicated that DEHP could affect hemocyte immune properties, and also cause non-specific general stress-response of the antioxidant complex, which, in turn, was not associated with pronounced oxidative stress.
贻贝分别暴露于邻苯二甲酸二（2-乙基己基）酯（DEHP）（0.4mg/L 和 4.0mg/L）24 小时和 48 小时，评估其对血细胞细胞组成和血细胞中自发性活性氧（ROS）水平的影响。暴露于 DEHP 诱导血细胞中自发性 ROS 产生水平降低，并减少血细胞中无颗粒细胞的数量。发现 DEHP 在贻贝的肝胰腺中积累，这一过程与孵育 24 小时后过氧化氢酶（CAT）活性增加有关。在实验期末（48 小时），CAT 活性恢复至对照水平。肝胰腺中超氧化物歧化酶（SOD）活性在暴露于 DEHP 48 小时后增加。结果表明，DEHP 可能会影响血细胞的免疫特性，并导致抗氧化复合物的非特异性一般应激反应，而这反过来与明显的氧化应激无关。
