Mincarelli Luana Fiorella, Turner Alexander, Anderson George, Wollenberg Valero Katharina
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", Via Campo Boario, 64100 Teramo, Italy.
School of Natural Sciences, University of Hull, Hull HU6 7RX, UK.
Toxics. 2024 Feb 24;12(3):172. doi: 10.3390/toxics12030172.
The endocrine disruptive chemical DEHP is a plasticiser often found in marine waters. Here, we assessed the effect of this additive on the number and size of eggs spawned by female mussels during a synchronised spawning event. After achieving the ripeness of the gonads, mussels of both sexes were exposed to two environmentally relevant concentrations of DEHP (nominal concentrations 0.5 and 50 µg/L) for one week. A spawning event was then induced and eggs were collected, counted, and their size measured (area and diameter). A slight but not significant effect was observed in lowering the number of eggs spawned when increasing the DEHP concentration. This effect was greater when adding spent gonads (possibly fully spawned females) to the total number of females. A significant effect of the lower dose on the average egg sizes was noticed, with a smaller area and diameter measured with respect to the control and the higher concentrated treatments. These results once again underline the importance for ecotoxicological studies to address the nonlinear dose-response effects of endocrine disruptive chemicals environmentally present at concentrations in the order of just a few µg/L that could not elicit a strong defence mechanism at low levels and be absorbed by filter feeder animals such as mussels.
内分泌干扰化学物质邻苯二甲酸二（2-乙基己基）酯（DEHP）是一种常在海水中发现的增塑剂。在此，我们评估了这种添加剂对雌性贻贝在同步产卵过程中所产卵子数量和大小的影响。性腺成熟后，将雌雄贻贝暴露于两种与环境相关的DEHP浓度（标称浓度为0.5和50μg/L）下一周。然后诱导产卵并收集卵子，进行计数并测量其大小（面积和直径）。当增加DEHP浓度时，观察到产卵数量略有下降，但不显著。当将已排卵的性腺（可能是完全产卵的雌性）加入雌性总数中时，这种影响更大。注意到较低剂量对平均卵子大小有显著影响，与对照和高浓度处理相比，测量到的面积和直径更小。这些结果再次强调了生态毒理学研究的重要性，即要研究环境中存在的内分泌干扰化学物质的非线性剂量-反应效应，这些物质的浓度仅为几μg/L，在低水平时可能不会引发强大的防御机制，却会被贻贝等滤食性动物吸收。
