College of Life Science, South-Central Minzu University, No. 182, Minyuan Road, Hongshan District, Wuhan City, Hubei Province 430074, China.
Hubei Province Nutrition Chemicals Biosynthetic Engineering Technology Research Center, Wuhan 430073, China.
Carbohydr Polym. 2023 Jul 15;312:120806. doi: 10.1016/j.carbpol.2023.120806. Epub 2023 Mar 12.
Alginate oligosaccharides (AOS) are divided by their monomer sequences into three types: oligomannuronate (MAOS), oligoguluronate (GAOS), and heterogeneous AOS (HAOS). However, how these AOS structures differentially regulate health and modulate gut microbiota is unclear. We explored the structure-function relationship of AOS both in an in vivo colitis model and an in vitro enterotoxigenic Escherichia coli (ETEC)-challenged cell model. We found that MAOS administration significantly alleviated the symptom of experimental colitis and improved the gut barrier function in vivo and in vivo. Nevertheless, HAOS and GAOS were less effective than MAOS. The abundance and diversity of gut microbiota are obviously increased by MAOS intervention, but not by HAOS or GAOS. Importantly, microbiota from MAOS-dosed mice through FMT decreased the disease index level, alleviated histopathological changes, and improved gut barrier function in the colitis model. Super FMT donors induced by MAOS but not by HAOS or GAOS, seemed to exert potential in colitis bacteriotherapy. These findings may aid in establishing precise pharmaceutical applications based on the targeted production of AOS.
藻酸盐寡糖（AOS）根据其单体序列分为三种类型：寡甘露糖醛酸（MAOS）、寡古罗糖醛酸（GAOS）和杂合 AOS（HAOS）。然而，这些 AOS 结构如何差异调节健康和调节肠道微生物群尚不清楚。我们在体内结肠炎模型和体外产肠毒素大肠杆菌（ETEC）挑战细胞模型中探索了 AOS 的结构-功能关系。我们发现 MAOS 给药可显著缓解实验性结肠炎的症状，并改善体内和体内的肠道屏障功能。然而，HAOS 和 GAOS 的效果不如 MAOS。MAOS 干预明显增加了肠道微生物群的丰度和多样性，但 HAOS 或 GAOS 则不然。重要的是，通过 FMT 从 MAOS 给药的小鼠的微生物群降低了疾病指数水平，减轻了组织病理学变化，并改善了结肠炎模型中的肠道屏障功能。由 MAOS 而不是 HAOS 或 GAOS 诱导的超级 FMT 供体似乎在结肠炎细菌治疗中具有潜力。这些发现可能有助于基于 AOS 的靶向生产建立精确的药物应用。
