Saha L, Mandal S, Choudhuri D K
Acta Physiol Hung. 1986;67(1):13-25.
Removal of the corpora allata from the emerging female adults of L. grandis caused a rapid and significant increase in the contents of total carbohydrate, glycogen, total lipids, cholesterol, total proteins and RNA in the fat body, and a significant drop in the contents of trehalose free fatty acids (FFA), phospholipids, free amino acids (FAA) and also in the activity of acid and alkaline phosphatase, glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) and exterase in the fat body, in comparison with the sham-operated controls. Treatment with juvenile hormone analogue (JHa) of the allatectomized insects significantly reverses the effects produced by allatectomy (P less than or equal to 0.01). Similarly, removal of the brain produced similar responses in the fat-body but with some exceptions such as the decrease of total protein and RNA and increase significant of FAA as compared to the sham-operated controls. Simultaneous removal of corpora-allata and brain produces a rapid increase in the contents of total carbohydrate, glycogen, total lipid, cholesterol, FAA and the activity of acid phosphatase in comparison with all other treatments performed in this study, while the contents of trehalose, phospholipid, FFA, total protein, RNA and the activity of GOT, GPT, general esterase and alkaline phosphatase in the fat-body decreased compared to all other treatments. The results obtained are discussed in relation to the neuro-endocrine control of metabolism in insects.
切除大蜡螟初羽化雌成虫的咽侧体，导致脂肪体中总碳水化合物、糖原、总脂质、胆固醇、总蛋白质和RNA的含量迅速且显著增加，与假手术对照组相比，脂肪体中游离海藻糖、游离脂肪酸（FFA）、磷脂、游离氨基酸（FAA）的含量以及酸性和碱性磷酸酶、谷氨酸草酰乙酸转氨酶（GOT）、谷氨酸丙酮酸转氨酶（GPT）和酯酶的活性显著下降。用保幼激素类似物（JHa）处理去咽侧体昆虫，可显著逆转去咽侧体所产生的影响（P≤0.01）。同样，切除脑在脂肪体中产生类似反应，但有一些例外，如与假手术对照组相比，总蛋白质和RNA减少，FAA显著增加。与本研究中的所有其他处理相比，同时切除咽侧体和脑会使总碳水化合物、糖原、总脂质、胆固醇、FAA的含量以及酸性磷酸酶的活性迅速增加，而脂肪体中海藻糖、磷脂、FFA、总蛋白质、RNA的含量以及GOT、GPT、一般酯酶和碱性磷酸酶的活性与所有其他处理相比则下降。结合昆虫新陈代谢的神经内分泌控制对所得结果进行了讨论。
