Department of Urology, The Second Xiangya Hospital, Central South University, No.139 Renmin Road, Changsha, Hunan Province, 410011, China.
Department of Urology, The Second Xiangya Hospital, Central South University, No.139 Renmin Road, Changsha, Hunan Province, 410011, China.
Tissue Cell. 2023 Jun;82:102078. doi: 10.1016/j.tice.2023.102078. Epub 2023 Mar 24.
Prostate cancer (PCa) is a prevalent cause of morbidity and mortality. DHRS2-modified human umbilical cord mesenchymal stem cells-derived exosomes (hUC-MSCs-derived exos) function in PCa. We explored the mechanism of DHRS2-modified hUC-MSCs-derived exos in PCa cell malignant behaviors. DHRS2 expression levels in WPMY-1 cells and 4 PCa cell lines were detected by RT-qPCR and Western blot. 22Rv1/DU145 cells with high/low DHRS2 expression were selected to establish the low/high DHRS2 expression models by transfection. Cell proliferation and apoptosis were detected by CCK-8, colony formation assays, and flow cytometry. hUC-MSCs were identified by oil red O, alizarin staining, and flow cytometry. Exos were extracted from hUC-MSCs by ultracentrifugation and identified by transmission electron microscopy, Nano series-Nano-ZS, and Western blot. DU145 cells were selected for in vitro study to further study the effects of DHRS2-modified exos on cell proliferation and apoptosis. The effect of DHRS2-modified exos on cell cycle distribution was detected by flow cytometry. DHRS2 was repressed in PCa cells. DHRS2 overexpression suppressed PCa cell proliferation and promoted apoptosis. Exos were successfully isolated from hUC-MSC. DHRS2-modified hUC-MSCs-derived exos carried DHRS2 into PCa cells and blocked malignant behaviors. Briefly, DHRS2 was repressed in PCa cells. DHRS2-modified hUC-MSCs-derived exos blocked PCa cell proliferation and enhanced apoptosis.
前列腺癌（PCa）是发病率和死亡率的主要原因。DHRS2 修饰的人脐带间充质干细胞衍生的外泌体（hUC-MSCs 衍生的外泌体）在 PCa 中发挥作用。我们探讨了 DHRS2 修饰的 hUC-MSCs 衍生的外泌体在 PCa 细胞恶性行为中的作用机制。通过 RT-qPCR 和 Western blot 检测 WPMY-1 细胞和 4 种 PCa 细胞系中 DHRS2 的表达水平。通过转染选择 22Rv1/DU145 细胞中 DHRS2 高/低表达，建立低/高 DHRS2 表达模型。通过 CCK-8、集落形成实验和流式细胞术检测细胞增殖和凋亡。油红 O、茜素红染色和流式细胞术鉴定 hUC-MSCs。通过超速离心从 hUC-MSCs 中提取外泌体，并通过透射电子显微镜、Nano 系列-Nano-ZS 和 Western blot 进行鉴定。选择 DU145 细胞进行体外研究，以进一步研究 DHRS2 修饰的外泌体对细胞增殖和凋亡的影响。通过流式细胞术检测 DHRS2 修饰的外泌体对细胞周期分布的影响。DHRS2 在 PCa 细胞中受到抑制。DHRS2 过表达抑制 PCa 细胞增殖并促进凋亡。成功从 hUC-MSC 中分离出外泌体。DHRS2 修饰的 hUC-MSCs 衍生的外泌体将 DHRS2 带入 PCa 细胞并阻断恶性行为。简而言之，DHRS2 在 PCa 细胞中受到抑制。DHRS2 修饰的 hUC-MSCs 衍生的外泌体阻断 PCa 细胞增殖并增强凋亡。
