de Harven E, Soligo D
Am J Anat. 1986 Feb-Mar;175(2-3):277-87. doi: 10.1002/aja.1001750212.
A method is described and discussed that permits the specific labeling of the surface of prefixed cells with the colloidal gold marker viewed with the scanning electron microscope. Its value depends exclusively on the use of backscattered electron imaging. Its advantages include the possibility of preserving the surface features of the labeled cells, the ease with which specificity can be established, the possibility of making total counts of the labeled surface antigenic sites, and the possibility of achieving distinct labeling for two different antigens expressed on the surface of the same cell.
本文描述并讨论了一种方法，该方法可使用胶体金标记物对固定细胞的表面进行特异性标记，以便用扫描电子显微镜观察。其价值完全取决于背散射电子成像的应用。其优点包括保留标记细胞表面特征的可能性、确定特异性的简便性、对标记表面抗原位点进行总数计数的可能性，以及对同一细胞表面表达的两种不同抗原实现清晰标记的可能性。
