Department of Biomolecular Science, University of Urbino, Carlo Bo, Piazza del Risnascimento, 6, 61029 Urbino, PU, Italy; School of Food Science and Nutrition, University of Leeds, Leeds LS2 9JT, UK.
School of Food Science and Nutrition, University of Leeds, Leeds LS2 9JT, UK; Department of Cell Biology and Histology, University of Murcia, 30100 Murcia, Spain.
Int J Biol Macromol. 2023 Jun 15;240:124464. doi: 10.1016/j.ijbiomac.2023.124464. Epub 2023 Apr 14.
trans-Cinnamaldehyde, known for its bacterial anti-quorum sensing activity when applied at sublethal concentrations, has gained traction given its potential use against multidrug resistant bacteria. In this work, trans-cinnamaldehyde-loaded oil-in-water nanocapsules coated with chitosan, N,N,N-trimethyl chitosan chloride, N-(2-(N,N,N-trimethylammoniumyl)acetyl) chitosan chloride or N-(6-(N,N,N-trimethylammoniumyl)hexanoyl)chitosan chloride were obtained. All the formulated nanocapsules showed a Z-average hydrodynamic diameter ~ 160 nm and ζ-potential higher than +40 mV. N,N,N-trimethyl chitosan-coated oil-in-water nanocapsules showed the greatest trans-cinnamaldehyde association efficiency (99.3 ± 7.6) % and total payload release (88.6 ± 22.5) %, while N-(6-(N,N,N-trimethylammoniumyl)hexanoyl)chitosan chloride chitosan-coated oil-in-water nanocapsules were the only formulations stable in phosphate buffer saline PBS (pH 7.4) upon incubation at 37 °C for 24 h. Future work should address the stability of the developed nanocapsules in culture media and their biological performance.
反式肉桂醛在亚致死浓度下具有抗细菌群体感应活性，因此引起了人们的关注，因为它有可能对抗多药耐药菌。在这项工作中，获得了用壳聚糖、N，N，N-三甲基壳聚糖盐酸盐、N-（2-（N，N，N-三甲基铵基）乙酰基）壳聚糖盐酸盐或 N-（6-（N，N，N-三甲基铵基）己酰基）壳聚糖盐酸盐包被的负载反式肉桂醛的油包水纳米胶囊。所有配方的纳米胶囊均表现出约 160nm 的 Z 均水力直径和高于+40mV 的 ζ-电位。N，N，N-三甲基壳聚糖包被的油包水纳米胶囊显示出最大的反式肉桂醛结合效率（99.3±7.6）%和总载药量释放（88.6±22.5）%，而 N-（6-（N，N，N-三甲基铵基）己酰基）壳聚糖盐酸盐壳聚糖包被的油包水纳米胶囊是唯一在 37°C 孵育 24 小时后在磷酸盐缓冲盐水 PBS（pH 7.4）中稳定的制剂。未来的工作应该解决开发的纳米胶囊在培养基中的稳定性及其生物学性能。
