Li Tong, Jiang Yueli, Yang Xiaofan, Li Huiling, Gong Zhongjun, Qin Yifan, Zhang Jing, Lu Ruijie, Wei Guoshu, Wu Yuqing, Lu Chuantao
Henan Key Laboratory of Crop Pest Control, Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, China.
Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, Baoding, China.
Front Physiol. 2023 Mar 31;14:1172542. doi: 10.3389/fphys.2023.1172542. eCollection 2023.
Light is an important abiotic factor affecting insect behavior. In nature, linearly polarized light is common, but circularly polarized light is rare. Left circularly polarized (LCP) light is selectively reflected by the exocuticle of most scarab beetles, including . Despite our previous research showing that this visual signal probably mediates their mating behavior, the way in which it does so is not well elucidated. In this study, we investigated how LCP light affects not only mating behavior but also gene expression in this species using RNA-seq. The results indicated that disruption of LCP light reflection by females of probably affects the process by which males of search for mates. Furthermore, the RNA-seq results showed that genes of the environmental signaling pathways and also of several insect reproduction-related amino acid metabolic pathways were differentially expressed in groups exposed and not exposed to LCP light. This implies that reproduction is probably regulated by LCP light-induced stress. Herein, the results show that LCP light is probably perceived by males of the species, further mediating their mating behavior. However, this hypothesis needs future verification with additional samples.
光作为一种重要的非生物因素，会影响昆虫的行为。在自然界中，线偏振光很常见，但圆偏振光却很罕见。左旋圆偏振（LCP）光会被包括……在内的大多数金龟子甲虫的外表皮选择性反射。尽管我们之前的研究表明，这种视觉信号可能介导了它们的交配行为，但其具体作用方式尚未得到充分阐明。在本研究中，我们使用RNA测序技术，研究了LCP光如何不仅影响该物种的交配行为，还影响其基因表达。结果表明，……雌性个体对LCP光反射的干扰，可能会影响……雄性个体寻找配偶的过程。此外，RNA测序结果显示，在暴露于和未暴露于LCP光的组中，环境信号通路以及一些与昆虫繁殖相关的氨基酸代谢通路的基因存在差异表达。这意味着……的繁殖可能受LCP光诱导的应激调节。在此，结果表明该物种的雄性个体可能感知到LCP光，进而介导它们的交配行为。然而，这一假设需要未来用更多样本进行验证。
