• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

经皮穿刺引流术是治疗化脓性肝脓肿的基石。

Hypercapsule is the cornerstone of in inducing pyogenic liver abscess.

机构信息

Department of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai, China.

Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.

出版信息

Front Cell Infect Microbiol. 2023 Mar 31;13:1147855. doi: 10.3389/fcimb.2023.1147855. eCollection 2023.

DOI:10.3389/fcimb.2023.1147855
PMID:37065211
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10102340/
Abstract

PURPOSE

To investigate the mechanisms of -induced pyogenic liver abscess (PLA).

METHODS

Forty-three strains from PLAs and 436 from non-PLAs were collected. Their differences were compared for virulence genes and factors, sequence types, and serotypes. Virulence genes , , and + were deleted in NTUH-K2044. Various analyses, such as transmission electron microscopy, neutrophil killing tests, and mouse lethality tests, were used to confirm the consequent changes.

RESULTS

Differences were found between strains from PLA and non-PLA samples for virulence genes and factors, including metabolism genes ( and ), capsular polysaccharide (CPS)-synthesis channel gene (), CPS-regulating genes (, , and ), and siderophore genes ( and ). When was positive, the difference between PLA and non-PLA samples was only observed with . Δ, Δ, and ΔΔ strains reverted to hypovirulence. In the Kupffer cell stimulation assay, interleukin (IL)-6, IL-12, IL-10, and transforming growth factor-β secretions were found to be equivalent in NTUH-K2044, Δ, Δ, and ΔΔ groups. Lower IL-1β and higher tumor necrosis factor-α secretions were observed for Δ, Δ, and ΔΔ.

CONCLUSIONS

Hypercapsule production is the cornerstone of hypervirulence, regardless of exopolysaccharides. K1 -induced PLA may decrease core inflammatory cytokines rather than increase anti-inflammatory cytokines. Exopolysaccharides could also attenuate the inflammatory response to aid in the immune escape of .

摘要

目的

研究 - 诱导的化脓性肝脓肿（PLA）的机制。

方法

收集 43 株 PLA 株和 436 株非 PLA 株。比较它们的毒力基因和因子、序列型和血清型差异。在 NTUH-K2044 中缺失了毒力基因 、 、 和 +。使用透射电子显微镜、中性粒细胞杀伤试验和小鼠致死性试验等各种分析方法来确认随后的变化。

结果

PLA 和非 PLA 样本的 株之间在毒力基因和因子方面存在差异，包括代谢基因（ 和 ）、荚膜多糖（CPS）合成通道基因（）、CPS 调节基因（ 、 、和 ）和铁载体基因（ 和 ）。当 为阳性时，仅在 PLA 和非 PLA 样本之间观察到 。Δ 、Δ 和 ΔΔ 菌株的毒力减弱。在 Kupffer 细胞刺激试验中，发现 NTUH-K2044、Δ 、Δ 和 ΔΔ 组的白细胞介素（IL）-6、IL-12、IL-10 和转化生长因子-β分泌相当。Δ 、Δ 和 ΔΔ 组的 IL-1β 分泌较低，肿瘤坏死因子-α 分泌较高。

结论

高荚膜产生是高毒力的基石，与外多糖无关。K1 诱导的 PLA 可能会降低核心炎症细胞因子，而不是增加抗炎细胞因子。外多糖也可以减轻炎症反应，帮助 逃避免疫。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b4/10102340/5a14611b6cf7/fcimb-13-1147855-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b4/10102340/950e025df876/fcimb-13-1147855-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b4/10102340/1382a2f4ee64/fcimb-13-1147855-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b4/10102340/dc1f05d0076e/fcimb-13-1147855-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b4/10102340/5a14611b6cf7/fcimb-13-1147855-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b4/10102340/950e025df876/fcimb-13-1147855-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b4/10102340/1382a2f4ee64/fcimb-13-1147855-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b4/10102340/dc1f05d0076e/fcimb-13-1147855-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b4/10102340/5a14611b6cf7/fcimb-13-1147855-g004.jpg

相似文献

1
Hypercapsule is the cornerstone of in inducing pyogenic liver abscess.经皮穿刺引流术是治疗化脓性肝脓肿的基石。
Front Cell Infect Microbiol. 2023 Mar 31;13:1147855. doi: 10.3389/fcimb.2023.1147855. eCollection 2023.
2
Coexistence of c-rmpA with p-rmpA and p-rmpA2 rather than excessive siderophores confers higher virulence in K1 Klebsiella pneumoniae.c-rmpA与p-rmpA和p-rmpA2共存而非铁载体过量，赋予肺炎克雷伯菌K1型更高的毒力。
Pathology. 2023 Dec;55(7):1004-1012. doi: 10.1016/j.pathol.2023.07.007. Epub 2023 Sep 9.
3
Higher Virulence Renders K2 a Stable Share Among Those from Pyogenic Liver Abscess.更高的毒力使K2在化脓性肝脓肿患者中占据稳定份额。
Infect Drug Resist. 2024 Jan 25;17:283-291. doi: 10.2147/IDR.S442454. eCollection 2024.
4
Lipopolysaccharide O1 antigen contributes to the virulence in Klebsiella pneumoniae causing pyogenic liver abscess.脂多糖 O1 抗原有助于化脓性肝脓肿的肺炎克雷伯菌的毒力。
PLoS One. 2012;7(3):e33155. doi: 10.1371/journal.pone.0033155. Epub 2012 Mar 12.
5
The role of Klebsiella pneumoniae rmpA in capsular polysaccharide synthesis and virulence revisited.重新探讨肺炎克雷伯菌 rmpA 基因在荚膜多糖合成和毒力中的作用。
Microbiology (Reading). 2011 Dec;157(Pt 12):3446-3457. doi: 10.1099/mic.0.050336-0. Epub 2011 Sep 29.
6
Emergence of K1 ST23 and K2 ST65 hypervirulent as true pathogens with specific virulence genes in cryptogenic pyogenic liver abscesses Shiraz Iran.在伊朗设拉子的隐源性化脓性肝脓肿中，出现了携带特定毒力基因的 K1 ST23 和 K2 ST65 超毒力菌株，成为真正的病原体。
Front Cell Infect Microbiol. 2022 Aug 9;12:964290. doi: 10.3389/fcimb.2022.964290. eCollection 2022.
7
Identification of an immuno-dominant protein from Klebsiella pneumoniae strains causing pyogenic liver abscess: implication in serodiagnosis.从引起化脓性肝脓肿的肺炎克雷伯菌菌株中鉴定免疫显性蛋白：对血清学诊断的意义。
BMC Microbiol. 2014 Dec 21;14:321. doi: 10.1186/s12866-014-0321-4.
8
Molecular epidemiology and virulence factors of pyogenic liver abscess causing Klebsiella pneumoniae in China.中国化脓性肝脓肿致肺炎克雷伯菌的分子流行病学和毒力因子。
Clin Microbiol Infect. 2014 Nov;20(11):O818-24. doi: 10.1111/1469-0691.12664. Epub 2014 Jun 14.
9
Capsular Polysaccharide Is Involved in NLRP3 Inflammasome Activation by Klebsiella pneumoniae Serotype K1.荚膜多糖参与肺炎克雷伯菌血清型K1对NLRP3炎性小体的激活。
Infect Immun. 2015 Sep;83(9):3396-409. doi: 10.1128/IAI.00125-15. Epub 2015 Jun 15.
10
Clinical, microbiological, and molecular epidemiological characteristics of -induced pyogenic liver abscess in southeastern China.中国东南部 - 引起的化脓性肝脓肿的临床、微生物学和分子流行病学特征。
Antimicrob Resist Infect Control. 2019 Oct 29;8:166. doi: 10.1186/s13756-019-0615-2. eCollection 2019.

引用本文的文献

1
Endogenous endophthalmitis due to liver abscess: a retrospective study of clinical course, treatment pattern, and prognosis.肝脓肿所致内源性眼内炎：临床病程、治疗模式及预后的回顾性研究
Int J Ophthalmol. 2025 Aug 18;18(8):1553-1560. doi: 10.18240/ijo.2025.08.18. eCollection 2025.
2
K1 Klebsiella pneumoniae is more conserved than K2 for both virulence plasmid and chromosome.肺炎克雷伯菌K1型在毒力质粒和染色体方面比K2型更保守。
BMC Genomics. 2025 Jul 10;26(1):652. doi: 10.1186/s12864-025-11624-8.
3
Multidrug-resistant hypervirulent : an evolving superbug.

本文引用的文献

1
Pyogenic and Amebic Infections of the Liver.肝化脓性和阿米巴性感染。
Gastroenterol Clin North Am. 2020 Jun;49(2):361-377. doi: 10.1016/j.gtc.2020.01.013.
2
Adaptive evolution of virulence and persistence in carbapenem-resistant Klebsiella pneumoniae.耐碳青霉烯类肺炎克雷伯菌毒力和持久性的适应性进化。
Nat Med. 2020 May;26(5):705-711. doi: 10.1038/s41591-020-0825-4. Epub 2020 Apr 13.
3
Protective Role of Kupffer Cells and Macrophages in Klebsiella pneumoniae-Induced Liver Abscess Disease.枯否细胞和巨噬细胞在肺炎克雷伯菌诱导的肝脓肿疾病中的保护作用。
多重耐药性高毒力：一种不断演变的超级细菌
Future Microbiol. 2025 Apr;20(6):499-511. doi: 10.1080/17460913.2025.2482478. Epub 2025 Mar 26.
4
Isoferulic acid facilitates effective clearance of hypervirulent Klebsiella pneumoniae through targeting capsule.异阿魏酸通过靶向荚膜促进高毒力肺炎克雷伯菌的有效清除。
PLoS Pathog. 2025 Jan 6;21(1):e1012787. doi: 10.1371/journal.ppat.1012787. eCollection 2025 Jan.
5
capsular polysaccharide: Mechanism in regulation of synthesis, virulence, and pathogenicity.荚膜多糖：合成、毒力和致病性调节机制
Virulence. 2024 Dec;15(1):2439509. doi: 10.1080/21505594.2024.2439509. Epub 2024 Dec 13.
6
Liver abscess induced by intestinal hypervirulent through down-regulation of tryptophan-IPA-IL22 axis.肠道高毒力菌株通过下调色氨酸-吲哚丙酸-白细胞介素22轴诱导肝脓肿。
iScience. 2024 Aug 30;27(10):110849. doi: 10.1016/j.isci.2024.110849. eCollection 2024 Oct 18.
7
Impact of LuxS on virulence and pathogenicity in exhibiting varied mucoid phenotypes.LuxS对呈现不同黏液样表型的菌株的毒力和致病性的影响。
Infect Immun. 2024 Mar 12;92(3):e0001224. doi: 10.1128/iai.00012-24. Epub 2024 Feb 15.
8
Sputum Gram stain of hypervirulent .
J Gen Fam Med. 2023 Aug 23;24(5):315-316. doi: 10.1002/jgf2.643. eCollection 2023 Sep.
Infect Immun. 2019 Aug 21;87(9). doi: 10.1128/IAI.00369-19. Print 2019 Sep.
4
Hypervirulent Klebsiella pneumoniae.高毒力肺炎克雷伯菌。
Clin Microbiol Rev. 2019 May 15;32(3). doi: 10.1128/CMR.00001-19. Print 2019 Jun 19.
5
Transcriptional regulation of galF by RcsAB affects capsular polysaccharide formation in Klebsiella pneumoniae NTUH-K2044.RcsAB 通过对 galF 的转录调控影响肺炎克雷伯菌 NTUH-K2044 荚膜多糖的形成。
Microbiol Res. 2018 Nov;216:70-78. doi: 10.1016/j.micres.2018.08.010. Epub 2018 Aug 25.
6
Identification of Two Regulators of Virulence That Are Conserved in Classical and Hypervirulent Strains.鉴定两种毒力调节因子，它们在经典和超强毒力菌株中保守存在。
mBio. 2018 Aug 7;9(4):e01443-18. doi: 10.1128/mBio.01443-18.
7
Identification of Biomarkers for Differentiation of Hypervirulent Klebsiella pneumoniae from Classical K. pneumoniae.鉴定高毒力肺炎克雷伯菌与经典肺炎克雷伯菌的生物标志物。
J Clin Microbiol. 2018 Aug 27;56(9). doi: 10.1128/JCM.00776-18. Print 2018 Sep.
8
A fatal outbreak of ST11 carbapenem-resistant hypervirulent Klebsiella pneumoniae in a Chinese hospital: a molecular epidemiological study.中国医院中致命的 ST11 碳青霉烯类耐药超毒力肺炎克雷伯菌爆发：一项分子流行病学研究。
Lancet Infect Dis. 2018 Jan;18(1):37-46. doi: 10.1016/S1473-3099(17)30489-9. Epub 2017 Aug 29.
9
ASK2 Bioactive Compound Inhibits MDR by Antibiofilm Activity, Modulating Macrophage Cytokines and Opsonophagocytosis.ASK2生物活性化合物通过抗生物膜活性、调节巨噬细胞细胞因子和调理吞噬作用来抑制多药耐药。
Front Cell Infect Microbiol. 2017 Aug 4;7:346. doi: 10.3389/fcimb.2017.00346. eCollection 2017.
10
Biliary diseases as main causes of pyogenic liver abscess caused by extended-spectrum beta-lactamase-producing Enterobacteriaceae.胆道疾病是产超广谱β-内酰胺酶肠杆菌科细菌所致化脓性肝脓肿的主要病因。
Liver Int. 2017 May;37(5):727-734. doi: 10.1111/liv.13267. Epub 2016 Oct 29.