Probst I, Spahr R, Schweickhardt C, Hunneman D H, Piper H M
Am J Physiol. 1986 May;250(5 Pt 2):H853-60. doi: 10.1152/ajpheart.1986.250.5.H853.
The energy metabolism of exogenous and endogenous substrates was investigated in Ca-tolerant adult ventricular myocytes in short-term culture. CO2 production from exogenous glucose (5 mM), lactate (5 mM), and palmitate (100 microM) were 8, 20, and 29 mumol X h-1 X g wet wt-1. Endogenous lipolysis shared in energy production by 78, 61, and 31%, respectively. Fatty acids represented the main respiratory fuel even when 10(-7) M insulin, 5 mM lactate, or 5 mM dichloroacetate were supplied in addition to glucose. With palmitate, triglyceride contents were doubled within 3 h of cell incubation. With 5 mM glucose as sole exogenous substrate the energy turnover in these resting cells resembled that of arrested hearts, corresponding to 2 ml O2 X min-1 X 100 g wet wt-1. It is concluded that the basic features of substrate metabolism resemble those of the intact myocardium in that fatty acids represent the major fuel, but the contribution of endogenous lipolysis is greater than in the beating heart.
在短期培养的耐钙成年心室肌细胞中研究了外源性和内源性底物的能量代谢。外源性葡萄糖（5 mM）、乳酸（5 mM）和棕榈酸（100 μM）产生的二氧化碳分别为8、20和29 μmol·h⁻¹·g湿重⁻¹。内源性脂解在能量产生中的占比分别为78%、61%和31%。即使除葡萄糖外还添加了10⁻⁷ M胰岛素、5 mM乳酸或5 mM二氯乙酸，脂肪酸仍是主要的呼吸燃料。使用棕榈酸时，细胞孵育3小时内甘油三酯含量增加了一倍。以5 mM葡萄糖作为唯一的外源性底物时，这些静息细胞中的能量转换类似于停搏心脏，相当于2 ml O₂·min⁻¹·100 g湿重⁻¹。结论是，底物代谢的基本特征与完整心肌相似，即脂肪酸是主要燃料，但内源性脂解的贡献大于跳动的心脏。
