Ichinohasama R, Hirokawa K, Hatakeyama S
Am J Reprod Immunol Microbiol. 1986 Apr;10(4):158-65. doi: 10.1111/j.1600-0897.1986.tb00017.x.
Two kinds of hybridoma clones, one producing monoclonal antibodies against Sertoli cell (TM-1) and the other the basal lamina of the seminiferous tubule (TM-2), were raised by fusion between P3X63Ag8-653 mouse myeloma cells and spleen cells of BALB/c mice (H-2d) immunized with testicular homogenate of the same inbred mice. Immunohistochemically, TM-1 reacted specifically with cytoplasmic component of Sertoli cell and TM-2 with basal lamina of the seminiferous tubule. Using these monoclonal antibodies, spermatogenic disturbance was induced experimentally in BDF1 (H-2b/d) by intratesticular injection of a set of these two antibodies. Single injection of either TM-1 or TM-2 failed to induce the lesion. This fact indicated that TM-1 antibody could reach the Sertoli cell to impair its function, which was otherwise inaccessible without coincidental action of TM-2 antibody. TM-2 antibody appeared to alter the permeability of the basal lamina of the tubule and lower its barrier effect.
通过将P3X63Ag8-653小鼠骨髓瘤细胞与用相同近交系小鼠睾丸匀浆免疫的BALB/c小鼠（H-2d）的脾细胞融合，获得了两种杂交瘤克隆，一种产生抗支持细胞的单克隆抗体（TM-1），另一种产生抗生精小管基膜的单克隆抗体（TM-2）。免疫组织化学显示，TM-1与支持细胞的细胞质成分特异性反应，TM-2与生精小管的基膜反应。使用这些单克隆抗体，通过在BDF1（H-2b/d）睾丸内注射这两种抗体的组合，实验性地诱导了生精障碍。单独注射TM-1或TM-2均未能诱导出病变。这一事实表明，TM-1抗体可以到达支持细胞并损害其功能，否则在没有TM-2抗体协同作用的情况下是无法实现的。TM-2抗体似乎改变了小管基膜的通透性并降低了其屏障作用。
