在印度比哈尔邦，使用改良的格雷斯昆虫培养基对杜氏利什曼原虫杜氏亚种进行初次分离。

Use of modified Grace's insect medium for the primary isolation of Leishmania donovani donovani in Bihar, India.

作者信息

Saran R, Gupta A K, Shrivastava S N, Prasad L S

出版信息

Am J Trop Med Hyg. 1986 May;35(3):488-90. doi: 10.4269/ajtmh.1986.35.488.

DOI:10.4269/ajtmh.1986.35.488

Abstract

Grace's insect medium, supplemented with 20% (v/v) heat-inactivated fetal bovine serum (FBS) and 15% defibrinated pooled rabbit blood is statistically shown to be more sensitive than modified NNN medium (P less than 0.05), Grace's insect medium with 20% (v/v) and Grace's insect medium with 30% (v/v) FBS (P less than 0.01) for in vitro primary isolation of promastigotes of Leishmania donovani donovani from cultures of bone marrow aspirates. This medium has been found to be especially useful for parasitological diagnosis of untreated cases of visceral leishmaniasis and for cases where microscopic examination of bone marrow aspirates has been negative for Leishman-Donovan bodies.

摘要

格雷斯昆虫培养基，添加20%（体积/体积）热灭活胎牛血清（FBS）和15%去纤维蛋白混合兔血，经统计学显示，对于从骨髓穿刺物培养物中体外初次分离杜氏利什曼原虫前鞭毛体，其比改良NNN培养基（P小于0.05）、含20%（体积/体积）FBS的格雷斯昆虫培养基以及含30%（体积/体积）FBS的格雷斯昆虫培养基更敏感（P小于0.01）。已发现该培养基对于未经治疗的内脏利什曼病病例以及骨髓穿刺物显微镜检查利什曼-多诺万小体呈阴性的病例的寄生虫学诊断特别有用。