Rassam M B, Al-Mudhaffar S A
Ann Trop Med Parasitol. 1979 Aug;73(4):345-7. doi: 10.1080/00034983.1979.11687268.
Leishmania donovani was isolated by culturing bone marrow aspirates from kala-azar patients, on three types of culture media. The isolation was successful in 88% of the cases. The effects of pH, D-glucose, L-proline and antibiotic concentration in the isolation were investigated. The semi-solid medium used (pH 7.4) with L-proline, was better than the modified NNN media for the primary isolation.
通过在三种培养基上培养黑热病患者的骨髓穿刺液来分离杜氏利什曼原虫。在88%的病例中分离成功。研究了pH值、D-葡萄糖、L-脯氨酸和抗生素浓度在分离过程中的作用。所使用的半固体培养基（pH 7.4）加L-脯氨酸，在初次分离时比改良的NNN培养基更好。
