Redinbaugh M G, Turley R B
Anal Biochem. 1986 Mar;153(2):267-71. doi: 10.1016/0003-2697(86)90091-6.
The bincinchoninic acid protein assay has been adapted for use with microtiter plates and a plate reader to reduce the time needed for analyses. The total volume of the assay was reduced to 0.21 ml with various ratios of sample to reagent volume being used. The assay is sensitive to a limit of less than 1 microgram of bovine serum albumin. The assay is insensitive to the presence of the detergents sodium dodecyl sulfate, Triton X-100, and deoxycholate. In addition, the method has been adapted for determining the protein concentrations of sucrose density gradient fractions, which eliminates the need for removing the sucrose prior to assay.
二辛可宁酸蛋白质测定法已适用于微孔板和酶标仪，以减少分析所需时间。通过使用不同的样品与试剂体积比，测定的总体积降至0.21毫升。该测定法对牛血清白蛋白的检测限低于1微克。该测定法对十二烷基硫酸钠、吐温X-100和脱氧胆酸盐等去污剂不敏感。此外，该方法已适用于测定蔗糖密度梯度级分的蛋白质浓度，从而无需在测定前去除蔗糖。
