Williams T D, Vachon L, Anderegg R J
Anal Biochem. 1986 Mar;153(2):372-9. doi: 10.1016/0003-2697(86)90105-3.
We report the negative ion chemical ionization mass spectra of 2,4-dinitrophenyl (DNP) amino acid methyl esters. For the common amino acids, these derivatives exhibit very simple mass spectra; the molecular anion is the base peak in all cases. The electrophilicity of the DNP group allows selective and sensitive ionization. Amino acids can be identified singly or in mixtures by their molecular weights, and picomole detection is possible. Chromatography is only needed for Leu/Ile differentiation. Amino-terminal analysis of proteins is demonstrated.
我们报道了2,4-二硝基苯基（DNP）氨基酸甲酯的负离子化学电离质谱。对于常见氨基酸，这些衍生物呈现出非常简单的质谱；在所有情况下，分子阴离子都是基峰。DNP基团的亲电性允许选择性和灵敏的电离。氨基酸可以通过其分子量单独或混合进行鉴定，并且可以进行皮摩尔检测。仅在区分亮氨酸/异亮氨酸时需要色谱法。展示了蛋白质的氨基末端分析。
