School of Life Sciences, Department of Neuroscience, Brain Research Center, Shenzhen Key Laboratory of Gene Regulation and Systems Biology, Southern University of Science and Technology, Shenzhen 518055, Guangdong, China.
School of Microelectronics, Southern University of Science and Technology, Shenzhen 518055, Guangdong, China.
Lab Chip. 2023 May 2;23(9):2217-2227. doi: 10.1039/d2lc01183b.
Microfluidic chambers are powerful tools for studying axonal mRNA localization and translation in neurons. In addition to specific manipulation and measurements of axons, microfluidic chambers are used for collecting axonal materials to perform axonal transcriptome analysis. However, traditional bipartite and tripartite chambers have limitations either in purity or quantity of collected axons. Here, we improved the design of traditional chambers. Moreover, we developed two new quantitative chambers, multi-compartmental quantitative bipartite chamber (MQBC) and long quantitative tripartite chamber (LQTC). Compared with the traditional chambers, MQBC and LQTC could dramatically increase the efficiency in collecting axonal RNA. Finally, we applied these chambers to do comparative axon transcriptome analysis of different types of neurons. Thus, our newly designed quantitative chambers significantly improve axon collection efficiency and facilitate axonal transcriptome analysis.
微流控室是研究神经元轴突 mRNA 定位和翻译的有力工具。除了对轴突进行特定的操作和测量外，微流控室还用于收集轴突材料以进行轴突转录组分析。然而，传统的双室和三室室在收集的轴突纯度或数量上存在局限性。在这里，我们改进了传统室的设计。此外，我们开发了两种新的定量室，多腔定量双室 (MQBC) 和长定量三室 (LQTC)。与传统的腔室相比，MQBC 和 LQTC 可以显著提高收集轴突 RNA 的效率。最后，我们应用这些腔室对不同类型的神经元进行比较轴突转录组分析。因此，我们新设计的定量腔室显著提高了轴突收集效率，促进了轴突转录组分析。
