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Determination of low levels of 4-fluorophenylalanine incorporation into proteins.

作者信息

Gamcsik M P

出版信息

Anal Biochem. 1986 Apr;154(1):311-5. doi: 10.1016/0003-2697(86)90530-0.

DOI:10.1016/0003-2697(86)90530-0
PMID:3706732
Abstract

This paper describes a rapid method for the determination of low levels of 4-fluorophenylalanine biosynthetically incorporated into proteins. Precolumn derivatization with phenylisothiocyanate followed by reverse-phase high-performance liquid chromatography enables determination of the amino acid composition of the protein as well as accurate detection of a 1-3% substitution of phenylalanine by fluorophenylalanine.

摘要

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