Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
Microbiol Spectr. 2023 Jun 15;11(3):e0040923. doi: 10.1128/spectrum.00409-23. Epub 2023 Apr 17.
Endometritis is a common cow disease characterized by inflammation of endometrium, which leads to infertility or low fertility of cows and brings huge economic losses to the dairy industry. Tau interferon (IFN-τ) has many important biological functions, including an anti-inflammatory effect. The present study aimed to survey the effects of IFN-τ administration on gut microflora and body metabolism in mice with endometritis and to explore the potential relationship. The results indicated that IFN-τ obviously alleviated the damage and ultrastructural changes of mouse endometrium induced by Escherichia coli and enhanced tight junction protein's expression level. Through analysis by 16S rRNA gene sequencing, we found that IFN-τ, especially at 12 h, could regulate the composition of gut microbiota associated with , Staphylococcus, and in E. coli-induced mouse endometritis. Through histometabonomics, it was found that endometritis was related to 11 different metabolites and 4 potential metabolic pathways. These metabolites and metabolic pathways were major participants in metabolic pathways, cysteine and methionine metabolism, arachidonic acid metabolism, and pyrimidine metabolism. Correlation analysis of gut microbiota with uterine tissue metabolomics showed that changes in metabolic pathways might be affected by gut microbiota, such as in mouse endometritis. The above results indicated that the anti-inflammatory mechanism of IFN-τ might be reduction of the abundance of in the gut microbiota, affecting the expression level of important metabolites in uterine tissue and thus playing an anti-inflammatory role. The change in intestinal flora has been the focus of many disease studies in recent years, but the pathogenetic effect of interferon on endometritis is still unclear. The results of this study showed that IFN-τ alleviated the damage in mouse endometritis induced by and improved the endometrial tissue barrier. Its functional mechanism may be reduction of the abundance of in the intestinal microbiota, affecting the expression level of important metabolites in uterine tissue and thus playing an anti-inflammatory role.
子宫内膜炎是一种常见的奶牛疾病，其特征为子宫内膜炎症，导致奶牛不孕或低育，并给奶牛养殖业带来巨大的经济损失。τ干扰素（IFN-τ）具有许多重要的生物学功能，包括抗炎作用。本研究旨在调查 IFN-τ 给药对子宫内膜炎小鼠肠道微生物群和机体代谢的影响，并探讨其潜在关系。结果表明，IFN-τ 明显缓解了大肠杆菌引起的小鼠子宫内膜损伤和超微结构变化，增强了紧密连接蛋白的表达水平。通过 16S rRNA 基因测序分析发现，IFN-τ，特别是在 12 小时时，可以调节与大肠杆菌诱导的小鼠子宫内膜炎相关的肠道微生物群组成。通过组织代谢组学分析发现，子宫内膜炎与 11 种不同的代谢物和 4 种潜在的代谢途径有关。这些代谢物和代谢途径是代谢途径、半胱氨酸和蛋氨酸代谢、花生四烯酸代谢和嘧啶代谢的主要参与者。肠道微生物群与子宫组织代谢组学的相关性分析表明，代谢途径的变化可能受肠道微生物群的影响，如大肠杆菌诱导的小鼠子宫内膜炎中的 。上述结果表明，IFN-τ 的抗炎机制可能是减少肠道微生物群中 的丰度，影响子宫组织中重要代谢物的表达水平，从而发挥抗炎作用。近年来，肠道菌群的变化一直是许多疾病研究的焦点，但干扰素对子宫内膜炎的发病机制尚不清楚。本研究结果表明，IFN-τ 缓解了大肠杆菌诱导的小鼠子宫内膜炎的损伤，改善了子宫内膜组织屏障。其功能机制可能是减少肠道微生物群中 的丰度，影响子宫组织中重要代谢物的表达水平，从而发挥抗炎作用。
