Chalissery Jessica, Wilson Isabel, Rankin Catharine, Liang Joseph
Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia, Canada.
Department of Psychology, University of British Columbia, Vancouver, British Columbia, Canada.
MicroPubl Biol. 2023 Mar 31;2023. doi: 10.17912/micropub.biology.000760. eCollection 2023.
Scientists use Parafilm to seal cultures on Nematode Growth Media (NGM) petri plates for short-term storage to reduce the likelihood of contamination and improve moisture retention. However, we found that maintaining worms on plates wrapped with Parafilm can affect multiple behavioral metrics when assaying tap-habituation behavior using the Multi-Worm Tracker (MWT). Most notably, worms cultured on parafilm-wrapped NGM plates exhibited slower speed of initial response to tap followed by marked sensitization. These findings suggest that labs should be conscious of the possibility that Parafilm may induce behavioral changes in when conducting experiments.
科学家们使用Parafilm密封线虫生长培养基（NGM）培养皿上的培养物，用于短期保存，以降低污染可能性并提高保湿性。然而，我们发现，当使用多线虫追踪器（MWT）测定轻拍习惯化行为时，在包裹有Parafilm的培养皿上饲养线虫会影响多个行为指标。最值得注意的是，在包裹有Parafilm的NGM培养皿上培养的线虫对轻拍的初始反应速度较慢，随后出现明显的敏感化。这些发现表明，实验室在进行实验时应意识到Parafilm可能对线虫行为产生变化的可能性。
