Department of Histology and Embryology, Faculty of Medicine, Dokuz Eylül University, Izmir, Turkey.
Eur Rev Med Pharmacol Sci. 2023 Apr;27(7):3022-3032. doi: 10.26355/eurrev_202304_31936.
The aim of our study was to test if oral high hyaluronic acid (HMW-HA) administration was effective in contrasting induced preterm birth (PTB) in female Wistar rats.
A total of 24 pregnant rats were pretreated with placebo or low (2.5 mg/day)/high dose (5 mg/day) of HMW-HA (day 15) and then induced to delivery with mifepristone plus prostaglandin E2 (PGE2) (3 mg/100 µL + 0.5 mg/animal) on the 19th day of pregnancy. The delivery time was recorded and the messenger RNA (mRNA) levels of pro-inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin (IL)1β, IL-6] were detected in the uterine tissues by real-time polymerase chain reaction (real PCR). Immunohistochemistry was performed alongside.
Oral HMW-HA was well absorbed in the body and was able to significantly delay the timing of delivery and decrease mRNA synthesis of pro-inflammatory cytokines.
HMW-HA, by acting in the management of PTB, may represent a new approach to protecting physiological pregnancy.
本研究旨在测试口服高分子量透明质酸（HMW-HA）是否能有效对抗诱导的早产（PTB）。
24 只怀孕大鼠在第 15 天预先给予安慰剂或低剂量（2.5mg/天）/高剂量（5mg/天）的 HMW-HA，然后在妊娠第 19 天用米非司酮和前列腺素 E2（PGE2）（3mg/100μL+0.5mg/动物）诱导分娩。记录分娩时间，并通过实时聚合酶链反应（real PCR）检测子宫组织中促炎细胞因子[肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）1β、IL-6]的信使 RNA（mRNA）水平。同时进行免疫组织化学检测。
HMW-HA 能被机体很好地吸收，并能显著延迟分娩时间，降低促炎细胞因子的 mRNA 合成。
HMW-HA 通过作用于 PTB 的管理，可能为保护生理妊娠提供一种新方法。
