Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou, China.
Department of Orthopedics, Suzhou Municipal Hospital, Suzhou, China.
J Biomater Sci Polym Ed. 2023 Oct;34(14):2000-2020. doi: 10.1080/09205063.2023.2204779. Epub 2023 Apr 22.
The periosteum plays a key role in bone tissue regeneration, especially in the promotion and protection of new bones. However, among the bone repair materials, many biomimetic artificial periosteum lack the natural periosteal structure, stem cells, and immunoregulation required for bone regeneration. In this study, we used natural periosteum to produce acellular periosteum. To retain the appropriate cell survival structure and immunomodulatory proteins, we grafted the functional polypeptide SKP on the surface collagen of the periosteum an amide bond, providing the acellular periosteum with the ability to recruit mesenchymal stem cells. Thus, we developed a biomimetic periosteum (DP-SKP) with the ability to promote stem cell homing and immunoregulation . Compared to the blank and simple decellularized periosteum groups, DP-SKP was more conducive to stem cell adhesion, growth, and osteogenic differentiation . Additionally, compared with the other two groups, DP-SKP significantly promoted mesenchymal stem cell homing to the periosteal transplantation site, improved the bone immune microenvironment, and accelerated new lamellar bone formation in the critical size defect of rabbit skulls . Therefore, this acellular periosteum with a mesenchymal stem cell homing effect is expected to be used as an extracellular artificial periosteum in clinical practice.
骨膜在骨组织再生中起着关键作用，特别是在促进和保护新骨方面。然而，在骨修复材料中，许多仿生人工骨膜缺乏新骨再生所需的天然骨膜结构、干细胞和免疫调节功能。在本研究中，我们使用天然骨膜制备去细胞骨膜。为了保留适当的细胞存活结构和免疫调节蛋白，我们在骨膜表面胶原上接枝具有功能的 SKP 短肽（酰胺键），赋予去细胞骨膜募集间充质干细胞的能力。因此，我们开发了一种具有促进干细胞归巢和免疫调节功能的仿生骨膜（DP-SKP）。与空白组和单纯去细胞骨膜组相比，DP-SKP 更有利于干细胞的黏附、生长和成骨分化。此外，与另外两组相比，DP-SKP 显著促进了间充质干细胞向骨膜移植部位的归巢，改善了骨免疫微环境，加速了兔颅骨临界尺寸缺损处新板层骨的形成。因此，这种具有间充质干细胞归巢作用的去细胞骨膜有望在临床上用作细胞外人工骨膜。
