基于半菁骨架的近红外荧光探针用于检测 CES1 活性和评估农药毒性。
A near-infrared fluorescent probe based on a hemi-cyanine skeleton for detecting CES1 activity and evaluating pesticide toxicity.
机构信息
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116024, China.
Pharmaceutical Research Center, Second Affiliated Hospital, Dalian Medical University, Dalian, 116023, China.
出版信息
J Mater Chem B. 2023 Apr 26;11(16):3587-3591. doi: 10.1039/d3tb00292f.
A novel near-infrared (NIR) fluorescent probe CHC-CES1 based on a hemi-cyanine skeleton for detecting carboxylesterase 1 (CES1) activity was developed. Herein, CHC-CES1 could be specifically hydrolysed to CHC-COOH along with a significant NIR fluorescence signal enhancement at 670 nm. Systematic evaluation indicated that CHC-CES1 possessed an outstanding selectivity and sensitivity towards CES1, and possessed good chemical stability in complex biosamples. Finally, CHC-CES1 was successfully used for the real-time imaging of endogenous CES1 activity in living cells. Moreover, CHC-CES1 was applied to evaluate the inhibitory effects of various pesticides towards CES1, and visually revealed the inhibitory effect of combined residue pesticides.
一种基于半花菁骨架的新型近红外（NIR）荧光探针 CHC-CES1 被开发出来，用于检测羧酸酯酶 1（CES1）活性。在这里，CHC-CES1 可以特异性地水解为 CHC-COOH，同时在 670nm 处产生显著的近红外荧光信号增强。系统评价表明，CHC-CES1 对 CES1 具有出色的选择性和灵敏度，并且在复杂的生物样品中具有良好的化学稳定性。最后，CHC-CES1 成功地用于活细胞内内源性 CES1 活性的实时成像。此外，CHC-CES1 被用于评估各种农药对 CES1 的抑制作用，并直观地显示了混合残留农药的抑制作用。
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