Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Pest Manag Sci. 2023 Sep;79(9):3250-3261. doi: 10.1002/ps.7506. Epub 2023 May 2.
Panonychus citri is a globally dominant citrus plant pest mite. Pesticide-induced population resurgence is a concern for mite control. Exposure to sublethal concentrations of pesticides has stimulated reproduction and outbreak risks in many pests. Pyridaben, a mitochondrial electron transport inhibitor, has been frequently used worldwide in mite control. In the study, sublethal and transgenerational effects of pyridaben exposure on Pyr_Rs (resistant) and Pyr_Control (susceptible) strains were systematically investigated in both exposed parental generation (F ) and unexposed offspring generations (F and F ) by evaluating life-table and physiological parameters.
After exposure to pyridaben, the fecundity of both strains was significantly reduced in F generation while significantly induced in F generation. Interestingly, these effects also stimulated the fecundity of the F generation in Pyr_Control strain while no significant effects occurred for Pyr_Rs strain. The intrinsic rate of increase (r) and finite rate of increase (λ) were significantly decreased only in F generation of Pyr_Control strain after exposure treatment. Meanwhile, the population projection indicated a smaller population size in F generation of Pyr_Control strain while a population increase for Pyr_Rs strain after sublethal treatment. Subsequent detoxification enzyme assays indicated that only P450 activities in F generation were significantly activated by LC exposure to pyridaben in both strains. Significant downregulation of reproduction-related (Pc_Vg) genes was observed in the F generations of both strains. Significant upregulation of P450 (CYP4CL2) and Pc_Vg of the F generation in both strains suggested the presence of delayed hormesis effects on the reproduction and developed tolerance to pyridaben, although the effects did not last over a longer period (F generation).
These results provide evidence for transgenerational hormesis effects of low concentrations of pyridaben exposure that may lead to population increase and resurgence risks of resistant-mites in natural settings by stimulating reproduction. © 2023 Society of Chemical Industry.
柑橘全爪螨是一种全球优势柑橘植物害螨。杀虫剂诱导的种群复苏是螨类防治的一个关注点。在许多害虫中，接触亚致死浓度的杀虫剂会刺激其繁殖和爆发风险。哒螨灵是一种线粒体电子传递抑制剂，在螨类防治中已在全球范围内广泛使用。本研究系统评估了亚致死浓度哒螨灵暴露对 Pyr_Rs（抗性）和 Pyr_Control（敏感）品系的亲代世代（F ）和未暴露后代世代（F 和 F ）的寿命表和生理参数的亚致死和跨代效应。
暴露于哒螨灵后，两系的 F 代繁殖力显著降低，而 F 代繁殖力显著增加。有趣的是，这些效应还刺激了 Pyr_Control 系 F 代的繁殖力，而 Pyr_Rs 系则没有显著影响。暴露处理后，仅 Pyr_Control 系的 F 代的内禀增长率（r）和有限增长率（λ）显著降低。同时，种群预测表明，亚致死处理后 Pyr_Control 系的 F 代种群规模较小，而 Pyr_Rs 系的种群增加。随后的解毒酶测定表明，仅在两系的 F 代中，LC 暴露于哒螨灵可显著激活 P450 活性。两系 F 代的繁殖相关基因（Pc_Vg）均显著下调。两系 F 代的 P450（CYP4CL2）和 Pc_Vg 的显著上调表明，哒螨灵的低浓度暴露存在延迟的激素效应，可刺激繁殖并产生对哒螨灵的耐受性，尽管这种效应不会持续更长时间（F 代）。
这些结果为哒螨灵低浓度暴露的跨代激素效应提供了证据，这可能通过刺激繁殖导致抗性螨在自然环境中种群增加和复苏风险。© 2023 化学工业协会。
