Institute of Organic Chemistry, University of Duisburg-Essen, Universitätstrasse 7, 45117, Essen, Germany) E-mail: christoph.hirschhäuseruni-due.de.
Department of Molecular Biology II, University of Duisburg-Essen, Universitätstrasse 7, 45117, Essen, Germany.
Chembiochem. 2023 Aug 1;24(15):e202300296. doi: 10.1002/cbic.202300296. Epub 2023 Jul 12.
A transfection vector based on a peptide dendrimer (1) has been developed and its abilities for DNA binding and transport have been investigated. By attaching a fluorophore to the vector system (1*), several steps in the transfection process could be monitored directly. As DLS and AFM studies showed, the labeled vector 1* condensed DNA into tightly packed aggregates able to enter eukaryotic cells. Co-localization experiments revealed that the ligand/plasmid complex is taken up by the endosomal pathway followed by an endosomal escape or lysosomal degradation. Afterwards, the plasmid DNA seems to enter the nucleus due to a breakdown of the nuclear envelope during mitosis, as only cells that have recently undergone mitosis showed H2B-GFP expression.
已经开发出一种基于肽树状大分子（1）的转染载体，并研究了其 DNA 结合和运输能力。通过将荧光团连接到载体系统（1*）上，可以直接监测转染过程中的几个步骤。如 DLS 和 AFM 研究所示，标记的载体 1*将 DNA 凝聚成紧密堆积的聚集体，能够进入真核细胞。共定位实验表明，配体/质粒复合物通过内体途径被摄取，随后发生内体逃逸或溶酶体降解。之后，由于有丝分裂期间核膜破裂，质粒 DNA 似乎进入细胞核，因为只有最近经历有丝分裂的细胞才显示 H2B-GFP 表达。
