一种细菌及其在与其相关的有毒甲藻不同培养条件下的种群动态。
A bacterium and its population dynamics under different culture conditions of its associated toxic dinoflagellate .
作者信息
Wu Zhen, Yang Xiaohong, Lin Senjie, Lee Wai Hin, Lam Paul K S
机构信息
State Key Laboratory of Marine Pollution, City University of Hong Kong, Hong Kong, China.
Department of Chemistry, City University of Hong Kong, Hong Kong, China.
出版信息
Mar Life Sci Technol. 2021 Jun 1;3(4):542-551. doi: 10.1007/s42995-021-00102-1. eCollection 2021 Nov.
UNLABELLED
bacteria are known as symbionts of legumes for developing nodules on plant roots and fixing N for the host plants but unknown for associations with dinoflagellates. Here, we detected, isolated, and characterized a species from the marine toxic dinoflagellate culture. Its 16S rRNA gene (rDNA) is 99% identical to that of , and the affiliation is supported by the phylogenetic placement of its cell wall hydrolase -encoding gene (). Using quantitative PCR of 16S rDNA and , we found that the abundance of this bacterium increased during the late exponential growth phase of and under nitrogen limitation, suggesting potential physiological interactions between the dinoflagellate and the bacterium. This is the first report of dinoflagellate-associated bacterium, and its prevalence and ecological roles in dinoflagellate- relationships remain to be investigated in the future.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s42995-021-00102-1.
未标记
细菌作为豆科植物的共生体，可在植物根部形成根瘤并为宿主植物固定氮，但与甲藻的关联尚不清楚。在此，我们从海洋有毒甲藻培养物中检测、分离并鉴定了一个物种。其16S rRNA基因（rDNA）与[具体物种]的16S rRNA基因有99%的同一性，并且其细胞壁水解酶编码基因（[具体基因]）的系统发育定位支持了这种归属关系。通过对16S rDNA和[具体基因]进行定量PCR，我们发现这种细菌的丰度在甲藻的指数生长后期以及氮限制条件下有所增加，这表明甲藻与该细菌之间可能存在生理相互作用。这是关于与甲藻相关细菌的首次报道，其在甲藻关系中的普遍性和生态作用仍有待未来研究。
补充信息
在线版本包含可在10.1007/s42995-021-00102-1获取的补充材料。
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